Molecular Cloning and Characterization of Rat Estrogen Receptor CDNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Mar 25

PMID 3031601

Citations 73

Authors

S Koike

M Sakai

M Muramatsu

Affiliations

Soon will be listed here.

Abstract

A cDNA clone of rat uterus estrogen receptor (ER) has been isolated and sequenced. This clone contains a complete open reading frame encoding 600 amino acid residues which is 5 and 11 amino acids larger than the corresponding molecules of human and chicken, respectively. The molecular weight of this protein is calculated to be 67,029. When this clone was ligated to the pSV2 vector and transfected into COS7 cells, a protein was produced that had the same affinity to estrogen as rat uterus ER. This sequence shows 88% homology with human ER; 528 amino acids are identical and 14 amino acids are conservative substitutions. The comparison of rat, human and chicken ER sequences indicate the presence of three highly conserved regions suggesting that these regions play important roles in ER function. The putative DNA-binding domain is completely identical in rat, human and chicken. The C-terminal half region which is thought to be the estrogen binding domain is also highly conserved and is rich in hydrophobic amino acid residues. Southern blot analysis of genomic DNA with ER cDNA as a probe has shown that related sequences are present in the genome.

Citing Articles

Estrogen's sex-specific effects on ischemic cell death and estrogen receptor mRNA expression in rat cortical organotypic explants.

Trout A, McLouth C, Westberry J, Sengoku T, Wilson M Aging Brain. 2024; 5:100117.

PMID: 38650743 PMC: 11033203. DOI: 10.1016/j.nbas.2024.100117.

Immunohistochemical Detection of Estrogen Receptor-Beta (ERβ) with PPZ0506 Antibody in Murine Tissue: From Pitfalls to Optimization.

Schroder S, Tag C, Kessel J, Antonson P, Weiskirchen R Biomedicines. 2022; 10(12).

PMID: 36551855 PMC: 9775465. DOI: 10.3390/biomedicines10123100.

Sex Differences in Psychostimulant Abuse: Implications for Estrogen Receptors and Histone Deacetylases.

Torres O Genes (Basel). 2022; 13(5).

PMID: 35627277 PMC: 9140379. DOI: 10.3390/genes13050892.

Estrogen Receptors Alpha and Beta Mediate Synaptic Transmission in the PFC and Hippocampus of Mice.

Zhang M, Weiland H, Schofbanker M, Zhang W Int J Mol Sci. 2021; 22(3).

PMID: 33540803 PMC: 7867372. DOI: 10.3390/ijms22031485.

Differential Roles of Hypothalamic AVPV and Arcuate Kisspeptin Neurons in Estradiol Feedback Regulation of Female Reproduction.

Wang L, Moenter S Neuroendocrinology. 2019; 110(3-4):172-184.

PMID: 31466075 PMC: 7047625. DOI: 10.1159/000503006.

References

Vincent A, Colot H, Rosbash M . Sequence and structure of the serendipity locus of Drosophila melanogaster. A densely transcribed region including a blastoderm-specific gene. J Mol Biol. 1985; 186(1):149-66. DOI: 10.1016/0022-2836(85)90265-7. View

Kumar V, Green S, Staub A, Chambon P . Localisation of the oestradiol-binding and putative DNA-binding domains of the human oestrogen receptor. EMBO J. 1986; 5(9):2231-6. PMC: 1167105. DOI: 10.1002/j.1460-2075.1986.tb04489.x. View

Jost J, Moncharmont B, Jiricny J, Saluz H, Hertner T . In vitro secondary activation (memory effect) of avian vitellogenin II gene in isolated liver nuclei. Proc Natl Acad Sci U S A. 1986; 83(1):43-7. PMC: 322787. DOI: 10.1073/pnas.83.1.43. View

Yamamoto K . Steroid receptor regulated transcription of specific genes and gene networks. Annu Rev Genet. 1985; 19:209-52. DOI: 10.1146/annurev.ge.19.120185.001233. View

Kozak M . Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986; 44(2):283-92. DOI: 10.1016/0092-8674(86)90762-2. View

Greene G, Gilna P, Waterfield M, Baker A, Hort Y, Shine J . Sequence and expression of human estrogen receptor complementary DNA. Science. 1986; 231(4742):1150-4. DOI: 10.1126/science.3753802. View

Green S, Walter P, Kumar V, Krust A, Bornert J, Argos P . Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature. 1986; 320(6058):134-9. DOI: 10.1038/320134a0. View

Mizusawa S, Nishimura S, Seela F . Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res. 1986; 14(3):1319-24. PMC: 339506. DOI: 10.1093/nar/14.3.1319. View

Hartshorne T, BLUMBERG H, Young E . Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA. Nature. 1986; 320(6059):283-7. DOI: 10.1038/320283a0. View

10.

Krust A, Green S, Argos P, Kumar V, Walter P, Bornert J . The chicken oestrogen receptor sequence: homology with v-erbA and the human oestrogen and glucocorticoid receptors. EMBO J. 1986; 5(5):891-7. PMC: 1166879. DOI: 10.1002/j.1460-2075.1986.tb04300.x. View

11.

Miesfeld R, Rusconi S, Godowski P, Maler B, Okret S, Wikstrom A . Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA. Cell. 1986; 46(3):389-99. DOI: 10.1016/0092-8674(86)90659-8. View

12.

Jeltsch J, Krozowski Z, Gronemeyer H, Simpson R, Garnier J, Krust A . Cloning of the chicken progesterone receptor. Proc Natl Acad Sci U S A. 1986; 83(15):5424-8. PMC: 386299. DOI: 10.1073/pnas.83.15.5424. View

13.

Conneely O, SULLIVAN W, Toft D, Birnbaumer M, Cook R, Maxwell B . Molecular cloning of the chicken progesterone receptor. Science. 1986; 233(4765):767-70. DOI: 10.1126/science.2426779. View

14.

Aviv H, Leder P . Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972; 69(6):1408-12. PMC: 426713. DOI: 10.1073/pnas.69.6.1408. View

15.

Graham F, Van Der Eb A . A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973; 52(2):456-67. DOI: 10.1016/0042-6822(73)90341-3. View

16.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

17.

Wrange O, Gustafsson J . Separation of the hormone- and DNA-binding sites of the hepatic glucocorticoid receptor by means of proteolysis. J Biol Chem. 1978; 253(3):856-65. View

18.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

19.

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

20.

Carlstedt-Duke J, Okret S, Wrange O, Gustafsson J . Immunochemical analysis of the glucocorticoid receptor: identification of a third domain separate from the steroid-binding and DNA-binding domains. Proc Natl Acad Sci U S A. 1982; 79(14):4260-4. PMC: 346650. DOI: 10.1073/pnas.79.14.4260. View