A Guanine-flipping and Sequestration Mechanism for G-quadruplex Unwinding by RecQ Helicases

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Oct 12

PMID 30305632

Citations 35

Authors

Andrew F Voter

Yupeng Qiu

Ramreddy Tippana

Sua Myong

James L Keck

Affiliations

Soon will be listed here.

Abstract

Homeostatic regulation of G-quadruplexes (G4s), four-stranded structures that can form in guanine-rich nucleic acids, requires G4 unwinding helicases. The mechanisms that mediate G4 unwinding remain unknown. We report the structure of a bacterial RecQ DNA helicase bound to resolved G4 DNA. Unexpectedly, a guanine base from the unwound G4 is sequestered within a guanine-specific binding pocket. Disruption of the pocket in RecQ blocks G4 unwinding, but not G4 binding or duplex DNA unwinding, indicating its essential role in structure-specific G4 resolution. A novel guanine-flipping and sequestration model that may be applicable to other G4-resolving helicases emerges from these studies.

Citing Articles

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Eukaryotic Pif1 helicase unwinds G-quadruplex and dsDNA using a conserved wedge.

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Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase.

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