Meroterpenoid-Rich Fraction of the Ethanolic Extract from Suppressed Oxidative Stress Induced by -Butyl Hydroperoxide in HepG2 Cells

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2018 Oct 12

PMID 30304831

Citations 9

Authors

Sujin Lim

Misung Kwon

Eun-Ji Joung

Taisun Shin

Chul-Woong Oh

Jae Sue Choi

Hyeung-Rak Kim

Affiliations

Soon will be listed here.

Abstract

species have been reported to be a source of phytochemicals, with a wide range of biological activities. In this study, we evaluated the hepatoprotective effect of a meroterpenoid-rich fraction of the ethanolic extract from (MES) against -butyl hydroperoxide (-BHP)-treated HepG2 cells. Treatment with MES recovered the cell viability from the -BHP-induced oxidative damage in a dose-dependent manner. It suppressed the reactive oxygen species production, lipid peroxidation, and glutathione depletion in the -BHP-treated HepG2 cells. The activity of the antioxidants induced by -BHP, including superoxide dismutase (SOD) and catalase, was reduced by the MES treatment. Moreover, it increased the nuclear translocation of nuclear factor erythroid 2-related factor 2, leading to the enhanced activity of glutathione S transferase, and the increased production of heme oxygenase-1 and NAD(P)H:quinine oxidoreductase 1 in -BHP-treated HepG2 cells. These results demonstrate that the antioxidant activity of MES substituted the activity of the SOD and catalase, and induced the production of detoxifying enzymes, indicating that MES might be used as a hepatoprotectant against -BHP-induced oxidative stress.

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