Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism

Overview

Journal Front Pharmacol

Date 2018 Oct 10

PMID 30298003

Citations 4

Authors

Eram Fauzia

Tarun Kumar Barbhuyan

Amit Kumar Shrivastava

Manish Kumar

Paarth Garg

Mohsin Ali Khan

Avril A B Robertson

Syed Shadab Raza

Affiliations

Soon will be listed here.

Abstract

Ischemia-reperfusion (I/R)-related disorders, such as stroke, myocardial infarction, and peripheral vascular disease, are among the most frequent causes of disease and death. Tissue injury or death may result from the initial ischemic insult, primarily determined by the magnitude and duration of the interruption in blood supply and then by the subsequent reperfusion-induced damage. Various and models are currently available to study I/R mechanism in the brain and other tissues. However, thus far, no I/R model has been reported for understanding the I/R mechanisms and for faster drug screening. Here, we developed an Hook model of I/R by occluding and releasing the right vitelline artery of a chick embryo at 72 h of development. To validate the model and elucidate various underlying survival and death mechanisms, we employed imaging (Doppler blood flow imaging), biochemical, and blotting techniques and evaluated the cell death mechanism: autophagy and inflammation caused by I/R. In conclusion, the present model is useful in parallel with established and I/R models to understand the mechanisms of I/R development and its treatment.

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