Promoter and Nucleotide Sequences of the Zymomonas Mobilis Pyruvate Decarboxylase

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1987 Mar 1

PMID 3029037

Citations 56

Authors

T Conway

Y A Osman

J I Konnan

E M Hoffmann

L O Ingram

Affiliations

Soon will be listed here.

Abstract

DNA sequence analysis showed that pyruvate decarboxylase (one of the most abundant proteins in Zymomonas mobilis) contains 559 amino acids. The promoter for the gene encoding pyruvate decarboxylase was not recognized by Escherichia coli, although the cloned gene was expressed at relatively high levels under the control of alternative promoters. The promoter region did not contain sequences which could be identified as being homologous to the generalized promoter structure for E. coli. Hydropathy plots for the amino acid sequence indicated that pyruvate decarboxylase contains a large number of hydrophobic domains which may contribute to the thermal stability of this enzyme.

Citing Articles

A First Marine Vibrio Biocatalyst to Produce Ethanol from Alginate, which is a Rich Polysaccharide in Brown Macroalgal Biomass.

Inohara Y, Chunqi J, Mino S, Swabe T Curr Microbiol. 2023; 80(5):143.

PMID: 36941373 DOI: 10.1007/s00284-023-03250-y.

Genome-Scale Transcription-Translation Mapping Reveals Features of Zymomonas mobilis Transcription Units and Promoters.

Vera J, Ghosh I, Zhang Y, Hebert A, Coon J, Landick R mSystems. 2020; 5(4).

PMID: 32694125 PMC: 7566282. DOI: 10.1128/mSystems.00250-20.

Improvement of Acetaldehyde Production in by Engineering of Its Aerobic Metabolism.

Kalnenieks U, Balodite E, Strahler S, Strazdina I, Rex J, Pentjuss A Front Microbiol. 2019; 10:2533.

PMID: 31798541 PMC: 6868117. DOI: 10.3389/fmicb.2019.02533.

The isc gene cluster expression ethanol tolerance associated improves its ethanol production by organic acids flux redirection in the ethanologenic Escherichia coli KO11 strain.

Martinez-Alcantar L, Diaz-Perez A, Campos-Garcia J World J Microbiol Biotechnol. 2019; 35(12):189.

PMID: 31748890 DOI: 10.1007/s11274-019-2769-8.

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa.

Yin G, Xin X, Fu S, An M, Wu S, Chen X Sci Rep. 2017; 7:40611.

PMID: 28094349 PMC: 5240128. DOI: 10.1038/srep40611.

References

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

Proudfoot N, Shander M, Manley J, Gefter M, Maniatis T . Structure and in vitro transcription of human globin genes. Science. 1980; 209(4463):1329-36. DOI: 10.1126/science.6158093. View

Grosjean H, Fiers W . Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene. 1982; 18(3):199-209. DOI: 10.1016/0378-1119(82)90157-3. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Ullrich J . Structure-function relationships in pyruvate decarboxylase of yeast and wheat germ. Ann N Y Acad Sci. 1982; 378:287-305. DOI: 10.1111/j.1749-6632.1982.tb31203.x. View

Doi R, Wang L . Multiple procaryotic ribonucleic acid polymerase sigma factors. Microbiol Rev. 1986; 50(3):227-43. PMC: 373069. DOI: 10.1128/mr.50.3.227-243.1986. View

Dickson R, Abelson J, Barnes W, Reznikoff W . Genetic regulation: the Lac control region. Science. 1975; 187(4171):27-35. DOI: 10.1126/science.1088926. View

Swings J, De Ley J . The biology of Zymomonas. Bacteriol Rev. 1977; 41(1):1-46. PMC: 413995. DOI: 10.1128/br.41.1.1-46.1977. View

Osman Y, Ingram L . Mechanism of ethanol inhibition of fermentation in Zymomonas mobilis CP4. J Bacteriol. 1985; 164(1):173-80. PMC: 214226. DOI: 10.1128/jb.164.1.173-180.1985. View

10.

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

11.

DE BOER H, Comstock L, Hui A, Wong E, Vasser M . A hybrid promoter and portable Shine-Dalgarno regions of Escherichia coli. Biochem Soc Symp. 1983; 48:233-44. View

12.

Ikemura T . Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system. J Mol Biol. 1981; 151(3):389-409. DOI: 10.1016/0022-2836(81)90003-6. View

13.

Ikemura T, Ozeki H . Codon usage and transfer RNA contents: organism-specific codon-choice patterns in reference to the isoacceptor contents. Cold Spring Harb Symp Quant Biol. 1983; 47 Pt 2:1087-97. DOI: 10.1101/sqb.1983.047.01.123. View

14.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

15.

Luria S, DELBRUCK M . Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943; 28(6):491-511. PMC: 1209226. DOI: 10.1093/genetics/28.6.491. View

16.

Bialkowska-Hobrzanska H, Gilchrist C, Denhardt D . Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein. J Bacteriol. 1985; 164(3):1004-10. PMC: 219290. DOI: 10.1128/jb.164.3.1004-1010.1985. View

17.

Pustell J, Kafatos F . A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination. Nucleic Acids Res. 1984; 12(1 Pt 2):643-55. PMC: 321079. DOI: 10.1093/nar/12.1part2.643. View

18.

HAWLEY D, McClure W . Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983; 11(8):2237-55. PMC: 325881. DOI: 10.1093/nar/11.8.2237. View

19.

Kozak M . Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev. 1983; 47(1):1-45. PMC: 281560. DOI: 10.1128/mr.47.1.1-45.1983. View

20.

. Evidence for a coding pattern on the non-coding strand of the E. coli genome. Nucleic Acids Res. 1984; 12(5):2235-41. PMC: 318658. DOI: 10.1093/nar/12.5.2235. View