Improved Osmotic Tolerance and Ethanol Production of Ethanologenic Escherichia Coli by IrrE, a Global Regulator of Radiation-resistance of Deinococcus Radiodurans

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2010 Oct 21

PMID 20959988

Citations 18

Authors

Ruiqiang Ma

Ying Zhang

Haozhou Hong

Wei Lu

Min Lin

Ming Chen

Wei Zhang

Affiliations

Soon will be listed here.

Abstract

Successful fermentations to produce ethanol using ethanologenic Escherichia coli require tolerance to high concentrations of sugars. Here we demonstrate that irrE, encoding a regulatory protein for radiation-resistance in Deinococcus radiodurans, conferred improved osmotic stress tolerance to E. coli. Expression of the gene protected E. coli cells against 25% glucose or xylose, acid shock. It also markedly improved cellular viability, the transcriptional levels of trehalose biosynthetic genes (otsBA) and trehalose content in the IrrE-expressing strain compared with the control strain. IrrE expression also enhanced the expression levels and enzymatic activities of PDC and ADHB as well as ethanol production. Our results suggest that IrrE could potentially be used to improve osmotic stress tolerance and ethanol production in ethanologenic strains.

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References

Kim J, Alizadeh P, Harding T, Klionsky D . Disruption of the yeast ATH1 gene confers better survival after dehydration, freezing, and ethanol shock: potential commercial applications. Appl Environ Microbiol. 1996; 62(5):1563-9. PMC: 167930. DOI: 10.1128/aem.62.5.1563-1569.1996. View

Bothast R, Nichols N, Dien B . Fermentations with new recombinant organisms. Biotechnol Prog. 1999; 15(5):867-75. DOI: 10.1021/bp990087w. View

Kobayashi A, Hirakawa H, Hirata T, Nishino K, Yamaguchi A . Growth phase-dependent expression of drug exporters in Escherichia coli and its contribution to drug tolerance. J Bacteriol. 2006; 188(16):5693-703. PMC: 1540079. DOI: 10.1128/JB.00217-06. View

Yomano L, York S, Ingram L . Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production. J Ind Microbiol Biotechnol. 1998; 20(2):132-8. DOI: 10.1038/sj.jim.2900496. View

Earl A, Mohundro M, Mian I, Battista J . The IrrE protein of Deinococcus radiodurans R1 is a novel regulator of recA expression. J Bacteriol. 2002; 184(22):6216-24. PMC: 151961. DOI: 10.1128/JB.184.22.6216-6224.2002. View

Makarova K, Omelchenko M, Gaidamakova E, Matrosova V, Vasilenko A, Zhai M . Deinococcus geothermalis: the pool of extreme radiation resistance genes shrinks. PLoS One. 2007; 2(9):e955. PMC: 1978522. DOI: 10.1371/journal.pone.0000955. View

Gao G, Tian B, Liu L, Sheng D, Shen B, Hua Y . Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli. DNA Repair (Amst). 2003; 2(12):1419-27. DOI: 10.1016/j.dnarep.2003.08.012. View

Ingram L, Aldrich H, Borges A, Causey T, Martinez A, Morales F . Enteric bacterial catalysts for fuel ethanol production. Biotechnol Prog. 1999; 15(5):855-66. DOI: 10.1021/bp9901062. View

Ohta K, Beall D, Mejia J, Shanmugam K, Ingram L . Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl Environ Microbiol. 1991; 57(4):893-900. PMC: 182819. DOI: 10.1128/aem.57.4.893-900.1991. View

10.

Elbein A, Pan Y, Pastuszak I, Carroll D . New insights on trehalose: a multifunctional molecule. Glycobiology. 2003; 13(4):17R-27R. DOI: 10.1093/glycob/cwg047. View

11.

Miller E, Ingram L . Combined effect of betaine and trehalose on osmotic tolerance of Escherichia coli in mineral salts medium. Biotechnol Lett. 2006; 29(2):213-7. DOI: 10.1007/s10529-006-9226-0. View

12.

MACKENZIE K, Eddy C, Ingram L . Modulation of alcohol dehydrogenase isoenzyme levels in Zymomonas mobilis by iron and zinc. J Bacteriol. 1989; 171(2):1063-7. PMC: 209702. DOI: 10.1128/jb.171.2.1063-1067.1989. View

13.

Ho N, Chen Z, Brainard A . Genetically engineered Saccharomyces yeast capable of effective cofermentation of glucose and xylose. Appl Environ Microbiol. 1998; 64(5):1852-9. PMC: 106241. DOI: 10.1128/AEM.64.5.1852-1859.1998. View

14.

Conway T, Osman Y, Konnan J, Hoffmann E, Ingram L . Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J Bacteriol. 1987; 169(3):949-54. PMC: 211885. DOI: 10.1128/jb.169.3.949-954.1987. View

15.

Hua Y, Narumi I, Gao G, Tian B, Satoh K, Kitayama S . PprI: a general switch responsible for extreme radioresistance of Deinococcus radiodurans. Biochem Biophys Res Commun. 2003; 306(2):354-60. DOI: 10.1016/s0006-291x(03)00965-3. View

16.

Neale A, Scopes R, Kelly J, Wettenhall R . The two alcohol dehydrogenases of Zymomonas mobilis. Purification by differential dye ligand chromatography, molecular characterisation and physiological roles. Eur J Biochem. 1986; 154(1):119-24. DOI: 10.1111/j.1432-1033.1986.tb09366.x. View

17.

Jung Y, Park H . Antisense-mediated inhibition of acid trehalase (ATH1) gene expression promotes ethanol fermentation and tolerance in Saccharomyces cerevisiae. Biotechnol Lett. 2005; 27(23-24):1855-9. DOI: 10.1007/s10529-005-3910-3. View

18.

Purvis J, Yomano L, Ingram L . Enhanced trehalose production improves growth of Escherichia coli under osmotic stress. Appl Environ Microbiol. 2005; 71(7):3761-9. PMC: 1168978. DOI: 10.1128/AEM.71.7.3761-3769.2005. View

19.

Miller E, Ingram L . Sucrose and overexpression of trehalose biosynthetic genes (otsBA) increase desiccation tolerance of recombinant Escherichia coli. Biotechnol Lett. 2007; 30(3):503-8. DOI: 10.1007/s10529-007-9573-5. View

20.

Underwood S, Buszko M, Shanmugam K, Ingram L . Lack of protective osmolytes limits final cell density and volumetric productivity of ethanologenic Escherichia coli KO11 during xylose fermentation. Appl Environ Microbiol. 2004; 70(5):2734-40. PMC: 404403. DOI: 10.1128/AEM.70.5.2734-2740.2004. View