Q-switched 1064 nm Nd-Yag Nanosecond Laser Effects on Skin Barrier Function and on Molecular Rejuvenation Markers in Keratinocyte-fibroblasts Interaction

Overview

Journal Lasers Med Sci

Publisher Springer

Date 2018 Oct 3

PMID 30276490

Citations 14

Authors

Anna De Filippis

Brunella Perfetto

Luigi Pio Guerrera

Giovanni Oliviero

Adone Baroni

Affiliations

Soon will be listed here.

Abstract

Skin represents an interface between internal and external environment; it protects human body by regulating the water loss and the maintenance of body temperature, defending against irritant and pathogen agents, and against physical, chemical, and UV damage. It provides to essential physiological functions, such as the important antioxidant defense capacity; its protective/defensive function is performed by a high number of proteins, and shows important functions in maintenance of skin barrier homeostasis. Keratinocytes and fibroblasts play a pivotal role to determine or prevent skin aging in response to intrinsic or extrinsic stimuli, modulating cytokines and several biochemical factors. Non-ablative technologies are playing an increasing role in the management of skin aging, inducing a dermal remodeling without a visible epidermal damage. The objective of this study was to evaluate the effect of Q-switched 1064 Nd-YAG laser (Medlite Conbio C6 Nd-YAG laser, Cynosure USA) in skin barrier function, analyzing the constituents which are strongly altered in aging skin. Particularly, we evaluated the expression of filaggrin, TGase, HSP70, and aquaporins, on HaCaT cells. The expression of proinflammatory cytokines has been investigated too.As a second step of the study, we analyzed the modulation of the rejuvenation molecular markers on human skin fibroblasts (HDFs) stimulated with keratinocytes conditioned medium (KCM).Our results demonstrated that Q-switched 1064 nm Nd:YAG laser acts on the skin barrier function, increasing the expression of aquaporins, filaggrin, TGase, and HSP70, modulating the proinflammatory cytokines. In fibroblasts stimulated with keratinocytes conditioned medium (KCM) and irradiated with Q-switched 1064 nm Nd:YAG laser, we can observe a reduction of MMP-1 and an increase in procollagen, collagen type I, and elastin. Our results highlight that Q-switched 1064 nm Nd:YAG laser treatment could represent an effective weapon to fight skin aging.

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References

Brenneisen P, Wlaschek M, Wenk J, Blaudschun R, Hinrichs R, Dissemond J . Ultraviolet-B induction of interstitial collagenase and stromelyin-1 occurs in human dermal fibroblasts via an autocrine interleukin-6-dependent loop. FEBS Lett. 1999; 449(1):36-40. DOI: 10.1016/s0014-5793(99)00402-0. View

Debelle L, Alix A . The structures of elastins and their function. Biochimie. 1999; 81(10):981-94. DOI: 10.1016/s0300-9084(99)00221-7. View

Takahashi H, Aoki N, Nakamura S, Asano K, Ishida-Yamamoto A, Iizuka H . Cornified cell envelope formation is distinct from apoptosis in epidermal keratinocytes. J Dermatol Sci. 2000; 23(3):161-9. DOI: 10.1016/s0923-1811(00)00071-2. View

Goldberg D, Silapunt S . Histologic evaluation of a Q-switched Nd:YAG laser in the nonablative treatment of wrinkles. Dermatol Surg. 2001; 27(8):744-6. DOI: 10.1046/j.1524-4725.2001.00353.x. View

Fisher G, Choi H, Bata-Csorgo Z, Shao Y, Datta S, Wang Z . Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo. J Invest Dermatol. 2001; 117(2):219-26. DOI: 10.1046/j.0022-202x.2001.01432.x. View

Rumalla V, Borah G . Cytokines, growth factors, and plastic surgery. Plast Reconstr Surg. 2001; 108(3):719-33. DOI: 10.1097/00006534-200109010-00019. View

Ye J, Garg A, Calhoun C, Feingold K, Elias P, Ghadially R . Alterations in cytokine regulation in aged epidermis: implications for permeability barrier homeostasis and inflammation. I. IL-1 gene family. Exp Dermatol. 2002; 11(3):209-16. DOI: 10.1034/j.1600-0625.2002.110303.x. View

Hara M, Ma T, Verkman A . Selectively reduced glycerol in skin of aquaporin-3-deficient mice may account for impaired skin hydration, elasticity, and barrier recovery. J Biol Chem. 2002; 277(48):46616-21. DOI: 10.1074/jbc.M209003200. View

Gorti G, Ronson S, Koch R . Wound healing. Facial Plast Surg Clin North Am. 2004; 10(2):119-27. DOI: 10.1016/s1064-7406(02)00004-4. View

10.

Schmults C, Phelps R, Goldberg D . Nonablative facial remodeling: erythema reduction and histologic evidence of new collagen formation using a 300-microsecond 1064-nm Nd:YAG laser. Arch Dermatol. 2004; 140(11):1373-6. DOI: 10.1001/archderm.140.11.1373. View

11.

Zeeuwen P . Epidermal differentiation: the role of proteases and their inhibitors. Eur J Cell Biol. 2005; 83(11-12):761-73. DOI: 10.1078/0171-9335-00388. View

12.

Jansen P, Rosch R, Jansen M, Binnebosel M, Junge K, Alfonso-Jaume A . Regulation of MMP-2 gene transcription in dermal wounds. J Invest Dermatol. 2007; 127(7):1762-7. DOI: 10.1038/sj.jid.5700765. View

13.

Daamen W, Veerkamp J, van Hest J, van Kuppevelt T . Elastin as a biomaterial for tissue engineering. Biomaterials. 2007; 28(30):4378-98. DOI: 10.1016/j.biomaterials.2007.06.025. View

14.

Houk L, Humphreys T . Masers to magic bullets: an updated history of lasers in dermatology. Clin Dermatol. 2007; 25(5):434-42. DOI: 10.1016/j.clindermatol.2007.05.004. View

15.

Mehta R, Fitzpatrick R . Endogenous growth factors as cosmeceuticals. Dermatol Ther. 2007; 20(5):350-9. DOI: 10.1111/j.1529-8019.2007.00149.x. View

16.

Puizina-Ivic N . Skin aging. Acta Dermatovenerol Alp Pannonica Adriat. 2008; 17(2):47-54. View

17.

S Orringer J, Hammerberg C, Hamilton T, Johnson T, Kang S, Sachs D . Molecular effects of photodynamic therapy for photoaging. Arch Dermatol. 2008; 144(10):1296-302. DOI: 10.1001/archderm.144.10.1296. View

18.

Helbig D, Paasch U . Molecular changes during skin aging and wound healing after fractional ablative photothermolysis. Skin Res Technol. 2011; 17(1):119-28. DOI: 10.1111/j.1600-0846.2010.00477.x. View

19.

Ogden S, Dearman R, Kimber I, Griffiths C . The effect of ageing on phenotype and function of monocyte-derived Langerhans cells. Br J Dermatol. 2011; 165(1):184-8. PMC: 3178785. DOI: 10.1111/j.1365-2133.2011.10313.x. View

20.

Ye X, Wang L, Dang Y, Liu B, Zhao D . Investigation of the 1064 nm Q-switched Nd:YAG laser on collagen expression in an animal model. Photomed Laser Surg. 2012; 30(10):604-9. DOI: 10.1089/pho.2012.3221. View