Acquisition of Resistance to Carbapenem and Macrolide-mediated Quorum Sensing Inhibition by Via ICE

Overview

Journal Commun Biol

Specialty Biology

Date 2018 Oct 2

PMID 30271939

Citations 18

Authors

Yichen Ding

Jeanette W P Teo

Daniela I Drautz-Moses

Stephan C Schuster

Michael Givskov

Liang Yang

Affiliations

Soon will be listed here.

Abstract

can cause life-threatening infections in immunocompromised patients. The first-line agents to treat infections are carbapenems. However, the emergence of carbapenem-resistant strains greatly compromised the effectiveness of carbapenem treatment, which makes the surveillance on their spreading and transmission important. Here we characterized the full-length genomes of two carbapenem-resistant clinical isolates that are capable of producing New Delhi metallo-β-lactamase-1 (NDM-1). We show that is carried by a novel integrative and conjugative element (ICE) ICE , which also carries the macrolide resistance gene and the florfenicol resistance gene . By exogenously expressing in laboratory strains, we show that Msr(E) can abolish azithromycin-mediated quorum sensing inhibition in vitro and anti-Pseudomonas effect in vivo. We conclude that ICEs are important in transmitting carbapenem resistance, and that anti-virulence treatment of infections using sub-inhibitory concentrations of macrolides can be challenged by horizontal gene transfer.

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