Effect of Alternating Electrical Current on Denitrifying Bacteria in a Microbial Electrochemical System: Biofilm Viability and ATP Assessment

Overview

Journal Environ Sci Pollut Res Int

Publisher Springer

Specialties Environmental Health
Toxicology

Date 2018 Oct 1

PMID 30269283

Citations 6

Authors

Somayyeh Dehghani

Abbas Rezaee

Saman Hosseinkhani

Affiliations

Soon will be listed here.

Abstract

The present study considers the impact of the alternating electric current on the viability and biological activity of denitrifying bacteria in a microbial electrochemical system (MES). The bio-stimulation using low-frequency low-voltage alternating current (AC) was studied in terms of the adenosine triphosphate (ATP) level of bacteria, viability, morphological characteristics, cell size, and complexity. Apoptosis assays by flow cytometry revealed that 81-95% of the cells were non-apoptotic, and cell membrane damage occurred < 18%. The applied AC could affect the bacterial metabolic activity and ATP content in the denitrifying bacteria depending on characteristics of the alternating electric current. Scanning electron microscopy (SEM) analysis of cell morphology illustrated low cell deformations under AC stimulation. The obtained results revealed that the applied alternating electrical current could increase the metabolic activity of denitrifying bacteria, leading to a better denitrification. Graphical abstract ᅟ.

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