Brillouin Spectroscopy and Radiography for Assessment of Viscoelastic and Regenerative Properties of Mammalian Bones

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2018 Sep 29

PMID 30264554

Citations 20

Authors

Dana Akilbekova

Vyacheslav Ogay

Talgat Yakupov

Madina Sarsenova

Bauyrzhan Umbayev

Asset Nurakhmetov

Kairat Tazhin

Vladislav V Yakovlev

Zhandos N Utegulov

Affiliations

Soon will be listed here.

Abstract

Biomechanical properties of mammalian bones, such as strength, toughness, and plasticity, are essential for understanding how microscopic-scale mechanical features can link to macroscale bones' strength and fracture resistance. We employ Brillouin light scattering (BLS) microspectroscopy for local assessment of elastic properties of bones under compression and the efficacy of the tissue engineering approach based on heparin-conjugated fibrin (HCF) hydrogels, bone morphogenic proteins, and osteogenic stem cells in the regeneration of the bone tissues. BLS is noninvasive and label-free modality for probing viscoelastic properties of tissues that can give information on structure-function properties of normal and pathological tissues. Results showed that MCS and BPMs are critically important for regeneration of elastic and viscous properties, respectively, HCF gels containing combination of all factors had the best effect with complete defect regeneration at week nine after the implantation of bone grafts and that the bones with fully consolidated fractures have higher values of elastic moduli compared with defective bones.

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References

Sakiyama-Elbert S, Hubbell J . Development of fibrin derivatives for controlled release of heparin-binding growth factors. J Control Release. 2000; 65(3):389-402. DOI: 10.1016/s0168-3659(99)00221-7. View

van Gastel N, Torrekens S, Roberts S, Moermans K, Schrooten J, Carmeliet P . Engineering vascularized bone: osteogenic and proangiogenic potential of murine periosteal cells. Stem Cells. 2012; 30(11):2460-71. DOI: 10.1002/stem.1210. View

Morin C, Hellmich C, Henits P . Fibrillar structure and elasticity of hydrating collagen: a quantitative multiscale approach. J Theor Biol. 2012; 317:384-93. DOI: 10.1016/j.jtbi.2012.09.026. View

Margallo-Balbas E, Taroni P, Pifferi A, Koolstra J, v Ruijven L, French P . The impact of morphology on light transport in cancellous bone. Phys Med Biol. 2010; 55(17):4917-31. DOI: 10.1088/0031-9155/55/17/003. View

Svedbom A, Hernlund E, Ivergard M, Compston J, Cooper C, Stenmark J . Osteoporosis in the European Union: a compendium of country-specific reports. Arch Osteoporos. 2013; 8:137. PMC: 3880492. DOI: 10.1007/s11657-013-0137-0. View

Scarcelli G, Yun S . In vivo Brillouin optical microscopy of the human eye. Opt Express. 2012; 20(8):9197-202. PMC: 3500092. DOI: 10.1364/OE.20.009197. View

Aerssens J, Boonen S, Lowet G, Dequeker J . Interspecies differences in bone composition, density, and quality: potential implications for in vivo bone research. Endocrinology. 1998; 139(2):663-70. DOI: 10.1210/endo.139.2.5751. View

Pfeffer C, Olsen B, Ganikhanov F, Legare F . Imaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy. Biomed Opt Express. 2011; 2(5):1366-76. PMC: 3087593. DOI: 10.1364/BOE.2.001366. View

Petite H, Viateau V, Bensaid W, Meunier A, de Pollak C, Bourguignon M . Tissue-engineered bone regeneration. Nat Biotechnol. 2000; 18(9):959-63. DOI: 10.1038/79449. View

10.

Manda K, Wallace R, Xie S, Levrero-Florencio F, Pankaj P . Nonlinear viscoelastic characterization of bovine trabecular bone. Biomech Model Mechanobiol. 2016; 16(1):173-189. PMC: 5285425. DOI: 10.1007/s10237-016-0809-y. View

11.

Gautschi O, Frey S, Zellweger R . Bone morphogenetic proteins in clinical applications. ANZ J Surg. 2007; 77(8):626-31. DOI: 10.1111/j.1445-2197.2007.04175.x. View

12.

Nyman J, Leng H, Dong X, Wang X . Differences in the mechanical behavior of cortical bone between compression and tension when subjected to progressive loading. J Mech Behav Biomed Mater. 2009; 2(6):613-9. PMC: 2736133. DOI: 10.1016/j.jmbbm.2008.11.008. View

13.

Weiner S, Traub W . Bone structure: from angstroms to microns. FASEB J. 1992; 6(3):879-85. View

14.

Ballmann C, Thompson J, Traverso A, Meng Z, Scully M, Yakovlev V . Stimulated Brillouin Scattering Microscopic Imaging. Sci Rep. 2015; 5:18139. PMC: 4686920. DOI: 10.1038/srep18139. View

15.

Elsayad K, Werner S, Gallemi M, Kong J, Sanchez Guajardo E, Zhang L . Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging. Sci Signal. 2016; 9(435):rs5. DOI: 10.1126/scisignal.aaf6326. View

16.

Palombo F, Madami M, Stone N, Fioretto D . Mechanical mapping with chemical specificity by confocal Brillouin and Raman microscopy. Analyst. 2014; 139(4):729-33. DOI: 10.1039/c3an02168h. View

17.

Palombo F, Winlove C, Edginton R, Green E, Stone N, Caponi S . Biomechanics of fibrous proteins of the extracellular matrix studied by Brillouin scattering. J R Soc Interface. 2014; 11(101):20140739. PMC: 4223901. DOI: 10.1098/rsif.2014.0739. View

18.

Matsukawa M, Tsubota R, Kawabe M, Fukui K . Application of a micro-Brillouin scattering technique to characterize bone in the GHz range. Ultrasonics. 2013; 54(5):1155-61. DOI: 10.1016/j.ultras.2013.09.016. View

19.

Zhang J, Fiore A, Yun S, Kim H, Scarcelli G . Line-scanning Brillouin microscopy for rapid non-invasive mechanical imaging. Sci Rep. 2016; 6:35398. PMC: 5064313. DOI: 10.1038/srep35398. View

20.

Krishnan L, Priddy L, Esancy C, Li M, Stevens H, Jiang X . Hydrogel-based Delivery of rhBMP-2 Improves Healing of Large Bone Defects Compared With Autograft. Clin Orthop Relat Res. 2015; 473(9):2885-97. PMC: 4523508. DOI: 10.1007/s11999-015-4312-z. View