Histopathological Changes in Androgenized Ovaries Are Recovered by Melatonin Treatment

Overview

Journal Int J Exp Pathol

Publisher Wiley

Specialty Pathology

Date 2018 Sep 27

PMID 30256483

Citations 1

Authors

Bianca R de Souza

Luiz Gustavo de Almeida Chuffa

Vinicius Augusto Simao

Isabel C C Camargo

Affiliations

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Abstract

Nandrolone decanoate (ND) is a synthetic steroid, which promotes adverse effects on the ovarian tissue, and melatonin (MLT) exhibits a number of beneficial properties in the reproductive system. This study evaluated the general features of the ovarian tissue and the immunoexpression of sex steroid receptors in ND-treated rats that were submitted to short-term melatonin treatment. Adult rats received mineral oil (control group) and ND at doses of 7.5 mg/kg for 15 days (ND-treated group). The treatment with MLT (10mg/kg for 7 days) was given alone, before or in combination with ND. All ND-treated animals showed persistent dioestrus. In the androgenized groups that received MLT, ovarian morphology and size, and the number/area of corpora lutea were recovered. The number of healthy and atretic follicles was recovered when MLT was administered prior to ND; this was similar to the ovaries of control and MLT groups. There was a decrease in estrogen receptors immunostaining in the follicles of androgenized rats that were treated with MLT, and pretreatment with MLT reduced the expression of androgen receptor in atretic follicles and corpora lutea, when compared with ND-treated group. We conclude that MLT treatment recovered the histopathological aspects of the androgenized ovaries, and MLT pretreatment was the most effective.

Citing Articles

Comparative ovarian morphophysiology of rats and Zebrafish after exposure to nandrolone decanoate.

Cavalcante J, Martins S, Martins J, Lobato J, Sales Y, Bittencourt S Anim Reprod. 2025; 22(1):e20240046.

PMID: 39867302 PMC: 11758781. DOI: 10.1590/1984-3143-AR2024-0046.

Histopathological changes in androgenized ovaries are recovered by melatonin treatment.

de Souza B, de Almeida Chuffa L, Simao V, Camargo I Int J Exp Pathol. 2018; 99(4):158-171.

PMID: 30256483 PMC: 6157297. DOI: 10.1111/iep.12283.

References

Drummond A, Fuller P . Ovarian actions of estrogen receptor-β: an update. Semin Reprod Med. 2012; 30(1):32-8. DOI: 10.1055/s-0031-1299595. View

Bondi C, Alonso-Gonzalez C, Clafshenkel W, Kotlarczyk M, Dodda B, Sanchez-Barcelo E . The effect of estradiol, progesterone, and melatonin on estrous cycling and ovarian aromatase expression in intact female mice. Eur J Obstet Gynecol Reprod Biol. 2013; 174:80-5. DOI: 10.1016/j.ejogrb.2013.11.027. View

Bordbar H, Mesbah F, Talaei T, Dehghani F, Mirkhani H . Modulatory effect of gonadotropins on rats' ovaries after nandrolone decanoate administration: a stereological study. Iran J Med Sci. 2014; 39(1):44-50. PMC: 3895894. View

Takasaki A, Nakamura Y, Tamura H, Shimamura K, Morioka H . Melatonin as a new drug for improving oocyte quality. Reprod Med Biol. 2018; 2(4):139-144. PMC: 5904612. DOI: 10.1111/j.1447-0578.2003.00035.x. View

Belardin L, Simao V, Leite G, de Almeida Chuffa L, Camargo I . Dose-dependent effects and reversibility of the injuries caused by nandrolone decanoate in uterine tissue and fertility of rats. Birth Defects Res B Dev Reprod Toxicol. 2014; 101(2):168-77. DOI: 10.1002/bdrb.21104. View

Guney M, Oral B, Karahan N, Mungan T . Regression of endometrial explants in a rat model of endometriosis treated with melatonin. Fertil Steril. 2007; 89(4):934-42. DOI: 10.1016/j.fertnstert.2007.04.023. View

Karbalay-Doust S, Noorafshan A . Stereological estimation of ovarian oocyte volume, surface area and number: application on mice treated with nandrolone decanoate. Folia Histochem Cytobiol. 2012; 50(2):275-9. DOI: 10.5603/fhc.2012.0037. View

Simao V, Belardin L, Leite G, de Almeida Chuffa L, Camargo I . Effects of different doses of nandrolone decanoate on estrous cycle and ovarian tissue of rats after treatment and recovery periods. Int J Exp Pathol. 2015; 96(5):338-49. PMC: 4693558. DOI: 10.1111/iep.12144. View

Gayan-Ramirez G, Rollier H, Vanderhoydonc F, Verhoeven G, Gosselink R, Decramer M . Nandrolone decanoate does not enhance training effects but increases IGF-I mRNA in rat diaphragm. J Appl Physiol (1985). 2000; 88(1):26-34. DOI: 10.1152/jappl.2000.88.1.26. View

10.

de Souza B, de Almeida Chuffa L, Simao V, Camargo I . Histopathological changes in androgenized ovaries are recovered by melatonin treatment. Int J Exp Pathol. 2018; 99(4):158-171. PMC: 6157297. DOI: 10.1111/iep.12283. View

11.

Mostafavi S, Akhondzadeh S, Mohammadi M, Keshtkar A, Hosseini S, Eshraghian M . Role of Melatonin in Body Weight: A Systematic Review and Meta-Analysis. Curr Pharm Des. 2016; 23(23):3445-3452. DOI: 10.2174/1381612822666161129145618. View

12.

Chuffa L, Seiva F, Favaro W, Teixeira G, Amorim J, Mendes L . Melatonin reduces LH, 17 beta-estradiol and induces differential regulation of sex steroid receptors in reproductive tissues during rat ovulation. Reprod Biol Endocrinol. 2011; 9:108. PMC: 3161940. DOI: 10.1186/1477-7827-9-108. View

13.

Wu H, Liu C, Duan W, Xu S, He M, Chen C . Melatonin ameliorates bisphenol A-induced DNA damage in the germ cells of adult male rats. Mutat Res. 2013; 752(1-2):57-67. DOI: 10.1016/j.mrgentox.2013.01.005. View

14.

Tamura H, Takasaki A, Taketani T, Tanabe M, Kizuka F, Lee L . Melatonin as a free radical scavenger in the ovarian follicle. Endocr J. 2012; 60(1):1-13. DOI: 10.1507/endocrj.ej12-0263. View

15.

Romeu L, da Motta E, Maganhin C, Oshima C, Fonseca M, Barrueco K . Effects of melatonin on histomorphology and on the expression of steroid receptors, VEGF, and PCNA in ovaries of pinealectomized female rats. Fertil Steril. 2010; 95(4):1379-84. DOI: 10.1016/j.fertnstert.2010.04.042. View

16.

Chaves E, Pereira-Junior P, Fortunato R, Masuda M, Campos de Carvalho A, Pires de Carvalho D . Nandrolone decanoate impairs exercise-induced cardioprotection: role of antioxidant enzymes. J Steroid Biochem Mol Biol. 2006; 99(4-5):223-30. DOI: 10.1016/j.jsbmb.2006.01.004. View

17.

Camargo I, Leite G, Pinto T, Ribeiro-Paes J . Histopathologycal findings in the ovaries and uterus of albino female rats promoted by co-administration of synthetic steroids and nicotine. Exp Toxicol Pathol. 2014; 66(4):195-202. DOI: 10.1016/j.etp.2014.01.005. View

18.

Tarumi W, Itoh M, Suzuki N . Effects of 5α-dihydrotestosterone and 17β-estradiol on the mouse ovarian follicle development and oocyte maturation. PLoS One. 2014; 9(6):e99423. PMC: 4050053. DOI: 10.1371/journal.pone.0099423. View

19.

Costa E, Mendonca B . Clinical management of transsexual subjects. Arq Bras Endocrinol Metabol. 2014; 58(2):188-96. DOI: 10.1590/0004-2730000003091. View

20.

Voordouw B, Euser R, Verdonk R, Alberda B, de Jong F, DROGENDIJK A . Melatonin and melatonin-progestin combinations alter pituitary-ovarian function in women and can inhibit ovulation. J Clin Endocrinol Metab. 1992; 74(1):108-17. DOI: 10.1210/jcem.74.1.1727807. View