Parvalbumin Alters Mitochondrial Dynamics and Affects Cell Morphology

Overview

Journal Cell Mol Life Sci

Publisher Springer

Specialty Biology

Date 2018 Sep 27

PMID 30255402

Citations 10

Authors

Lucia Lichvarova

Thomas Henzi

Dzhamilja Safiulina

Allen Kaasik

Beat Schwaller

Affiliations

Soon will be listed here.

Abstract

The Ca-binding protein parvalbumin (PV) and mitochondria play important roles in Ca signaling, buffering and sequestration. Antagonistic regulation of PV and mitochondrial volume is observed in in vitro and in vivo model systems. Changes in mitochondrial morphology, mitochondrial volume and dynamics (fusion, fission, mitophagy) resulting from modulation of PV were investigated in MDCK epithelial cells with stable overexpression/downregulation of PV. Increased PV levels resulted in smaller, roundish cells and shorter mitochondria, the latter phenomenon related to reduced fusion rates and decreased expression of genes involved in mitochondrial fusion. PV-overexpressing cells displayed increased mitophagy, a likely cause for the decreased mitochondrial volumes and the smaller overall cell size. Cells showed lower mobility in vitro, paralleled by reduced protrusions. Constitutive PV down-regulation in PV-overexpressing cells reverted mitochondrial morphology and fractional volume to the state present in control MDCK cells, resulting from increased mitochondrial movement and augmented fusion rates. PV-modulated, bi-directional and reversible mitochondrial dynamics are key to regulation of mitochondrial volume.

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