RUP2 Represses UVR8-mediated Flowering in Noninductive Photoperiods

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2018 Sep 27

PMID 30254107

Citations 23

Authors

Adriana B Arongaus

Song Chen

Marie Pireyre

Nina Glockner

Vinicius C Galvao

Andreas Albert

J Barbro Winkler

Christian Fankhauser

Klaus Harter

Roman Ulm

Affiliations

Soon will be listed here.

Abstract

Plants have evolved complex photoreceptor-controlled mechanisms to sense and respond to seasonal changes in day length. This ability allows plants to optimally time the transition from vegetative growth to flowering. UV-B is an important part intrinsic to sunlight; however, whether and how it affects photoperiodic flowering has remained elusive. Here, we report that, in the presence of UV-B, genetic mutation of () renders the facultative long day plant a day-neutral plant and that this phenotype is dependent on the UV RESISTANCE LOCUS 8 (UVR8) UV-B photoreceptor. We provide evidence that the floral repression activity of RUP2 involves direct interaction with CONSTANS, repression of this key activator of flowering, and suppression of transcription. RUP2 therefore functions as an essential repressor of UVR8-mediated induction of flowering under noninductive short day conditions and thus provides a crucial mechanism of photoperiodic flowering control.

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References

Jaeger K, Wigge P . FT protein acts as a long-range signal in Arabidopsis. Curr Biol. 2007; 17(12):1050-4. DOI: 10.1016/j.cub.2007.05.008. View

Wenkel S, Turck F, Singer K, Gissot L, Le Gourrierec J, Samach A . CONSTANS and the CCAAT box binding complex share a functionally important domain and interact to regulate flowering of Arabidopsis. Plant Cell. 2006; 18(11):2971-84. PMC: 1693937. DOI: 10.1105/tpc.106.043299. View

Haring M, Offermann S, Danker T, Horst I, Peterhansel C, Stam M . Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization. Plant Methods. 2007; 3:11. PMC: 2077865. DOI: 10.1186/1746-4811-3-11. View

Tiwari S, Shen Y, Chang H, Hou Y, Harris A, Ma S . The flowering time regulator CONSTANS is recruited to the FLOWERING LOCUS T promoter via a unique cis-element. New Phytol. 2010; 187(1):57-66. DOI: 10.1111/j.1469-8137.2010.03251.x. View

Cloix C, Kaiserli E, Heilmann M, Baxter K, Brown B, OHara A . C-terminal region of the UV-B photoreceptor UVR8 initiates signaling through interaction with the COP1 protein. Proc Natl Acad Sci U S A. 2012; 109(40):16366-70. PMC: 3479605. DOI: 10.1073/pnas.1210898109. View

Zhang B, Wang L, Zeng L, Zhang C, Ma H . Arabidopsis TOE proteins convey a photoperiodic signal to antagonize CONSTANS and regulate flowering time. Genes Dev. 2015; 29(9):975-87. PMC: 4421985. DOI: 10.1101/gad.251520.114. View

Deng X, Matsui M, Wei N, Wagner D, Chu A, Feldmann K . COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G beta homologous domain. Cell. 1992; 71(5):791-801. DOI: 10.1016/0092-8674(92)90555-q. View

Alonso J, Stepanova A, Leisse T, Kim C, Chen H, Shinn P . Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003; 301(5633):653-7. DOI: 10.1126/science.1086391. View

Heijde M, Ulm R . Reversion of the Arabidopsis UV-B photoreceptor UVR8 to the homodimeric ground state. Proc Natl Acad Sci U S A. 2013; 110(3):1113-8. PMC: 3549095. DOI: 10.1073/pnas.1214237110. View

10.

Oravecz A, Baumann A, Mate Z, Brzezinska A, Molinier J, Oakeley E . CONSTITUTIVELY PHOTOMORPHOGENIC1 is required for the UV-B response in Arabidopsis. Plant Cell. 2006; 18(8):1975-90. PMC: 1533968. DOI: 10.1105/tpc.105.040097. View

11.

Gnesutta N, Kumimoto R, Swain S, Chiara M, Siriwardana C, Horner D . CONSTANS Imparts DNA Sequence Specificity to the Histone Fold NF-YB/NF-YC Dimer. Plant Cell. 2017; 29(6):1516-1532. PMC: 5502446. DOI: 10.1105/tpc.16.00864. View

12.

Takada S, Goto K . Terminal flower2, an Arabidopsis homolog of heterochromatin protein1, counteracts the activation of flowering locus T by constans in the vascular tissues of leaves to regulate flowering time. Plant Cell. 2003; 15(12):2856-65. PMC: 282816. DOI: 10.1105/tpc.016345. View

13.

Fiil B, Qiu J, Petersen K, Petersen M, Mundy J . Coimmunoprecipitation (co-IP) of Nuclear Proteins and Chromatin Immunoprecipitation (ChIP) from Arabidopsis. CSH Protoc. 2011; 2008:pdb.prot5049. DOI: 10.1101/pdb.prot5049. View

14.

Lau O, Deng X . The photomorphogenic repressors COP1 and DET1: 20 years later. Trends Plant Sci. 2012; 17(10):584-93. DOI: 10.1016/j.tplants.2012.05.004. View

15.

Turck F, Fornara F, Coupland G . Regulation and identity of florigen: FLOWERING LOCUS T moves center stage. Annu Rev Plant Biol. 2008; 59:573-94. DOI: 10.1146/annurev.arplant.59.032607.092755. View

16.

Brown B, Cloix C, Jiang G, Kaiserli E, Herzyk P, Kliebenstein D . A UV-B-specific signaling component orchestrates plant UV protection. Proc Natl Acad Sci U S A. 2005; 102(50):18225-30. PMC: 1312397. DOI: 10.1073/pnas.0507187102. View

17.

Ulm R, Baumann A, Oravecz A, Mate Z, Adam E, Oakeley E . Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis. Proc Natl Acad Sci U S A. 2004; 101(5):1397-402. PMC: 337064. DOI: 10.1073/pnas.0308044100. View

18.

Gonzalez Besteiro M, Bartels S, Albert A, Ulm R . Arabidopsis MAP kinase phosphatase 1 and its target MAP kinases 3 and 6 antagonistically determine UV-B stress tolerance, independent of the UVR8 photoreceptor pathway. Plant J. 2011; 68(4):727-37. DOI: 10.1111/j.1365-313X.2011.04725.x. View

19.

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

20.

Wang W, Yang D, Feldmann K . EFO1 and EFO2, encoding putative WD-domain proteins, have overlapping and distinct roles in the regulation of vegetative development and flowering of Arabidopsis. J Exp Bot. 2011; 62(3):1077-88. DOI: 10.1093/jxb/erq336. View