Nondefective Spleen Necrosis Virus-derived Vectors Define the Upper Size Limit for Packaging Reticuloendotheliosis Viruses

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Dec 1

PMID 3024171

Citations 20

Authors

C Gelinas

H M Temin

Affiliations

Soon will be listed here.

Abstract

We constructed a nondefective retrovirus vector based on spleen necrosis virus (SNV), a replication-competent reticuloendotheliosis virus. We introduced different DNA sequences into this vector and studied the ability of the resulting viruses to replicate in chicken embryo fibroblasts. The replication efficiency of SNV-derived viruses decreased with increasing virus size. Viruses larger than 9.4 kilobases (kb) were rapidly overgrown by replication-competent deletion mutants. The size restriction for the efficient replication of nondefective SNV-derived viruses prevented the production of viruses larger than 10.0 kb. Analysis of the kinetics of virus particle release indicated that the size restriction occurred during virus encapsidation.

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