New Procedure for DNA Transfection with Polycation and Dimethyl Sulfoxide

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1984 Jun 1

PMID 6330534

Citations 216

Authors

S Kawai

M Nishizawa

Affiliations

Soon will be listed here.

Abstract

A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to the cell surface and dimethyl sulfoxide as an agent to facilitate the uptake of adsorbed DNA by the cells. In this new, simple, and convenient polycation-dimethyl sulfoxide transfection, which requires no carrier DNA even with small amounts of DNA, the number of transformed cell foci induced by Rous sarcoma virus DNA was proportional to the dose of the transfecting DNA, and chicken embryo fibroblast cells were successfully transformed by v-src-containing subgenomic DNA as well.

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