MicroRNA-194-5p Levels Decrease During Deep Hypothermic Circulatory Arrest

Overview

Journal Sci Rep

Specialty Science

Date 2018 Sep 21

PMID 30232383

Citations 9

Authors

Xiaohua Wang

Zerong You

Guoguang Zhao

Tianlong Wang

Affiliations

Soon will be listed here.

Abstract

Hypothermia has been reported to be effective in protecting the brain in various clinical conditions, including resuscitation after cardiac arrest and complex cardiovascular surgery, and is considered to be a promising therapy for stroke. The present study aimed to confirm the pivotal role that miRNA-194-5p plays in deep hypothermia circulation arrest. On the basis of reductions in expression of miR-194-5p in the circulation of 21 aortic dissection patients who underwent deep hypothermia circulatory arrest, the specific expression, target, and function of miR-194-5p was investigated using primary neuron culture, polymerase chain reaction, in situ hybridization, and flow cytometry methods. Our results showed that miR-194-5p expression was significantly downregulated in hypothermia oxygen glucose deprivation-treated neurons in vitro. Cortical neurons transfected with miR-194-5p mimic exhibited increased death due to oxygen-glucose deprivation. MiR-194-5p mediated the regulation of neuronal death, which involves the downregulation of the specific target protein SUMO2, which is crucial to ischemia tolerance. Collectively, these data highlight the unique role of miR-194-5p in mediating the deep hypothermia circulation arrest response via the regulation of SUMO2. These findings suggest that miR-194-5p could be a potential therapeutic target for intervention in ischemic disease.

Citing Articles

Bioinformatic analysis identifies GPR91 as a potential key gene in brain injury after deep hypothermic low flow.

Puwei S, Jiali X, Zhuoga D, Kede W, Patel N, Jia A Heliyon. 2023; 9(5):e15286.

PMID: 37187908 PMC: 10176032. DOI: 10.1016/j.heliyon.2023.e15286.

miR194 hypomethylation regulates coronary artery disease pathogenesis.

Duan L, Liu Y, Li J, Zhang Y, Liao J, Dong Y BMC Med Genomics. 2022; 15(1):264.

PMID: 36529725 PMC: 9759901. DOI: 10.1186/s12920-022-01421-7.

LINC00452 overexpression reverses oxLDL-induced injury of human umbilical vein endothelial cells (HUVECs) regulating miR-194-5p/IGF1R axis.

Yuan L, Wang D, Zhou Z Front Cardiovasc Med. 2022; 9:975640.

PMID: 36158838 PMC: 9500390. DOI: 10.3389/fcvm.2022.975640.

microRNAs Control Antiviral Immune Response, Cell Death and Chemotaxis Pathways in Human Neuronal Precursor Cells (NPCs) during Zika Virus Infection.

Polonio C, da Silva P, Russo F, Hyppolito B, Zanluqui N, Benazzato C Int J Mol Sci. 2022; 23(18).

PMID: 36142200 PMC: 9499039. DOI: 10.3390/ijms231810282.

Construction of five microRNAs prognostic markers and a prognostic model for clear cell renal cell carcinoma.

Zhou Q, Zhang Z, Ang X, Hu C, Ouyang J Transl Cancer Res. 2022; 10(5):2337-2353.

PMID: 35116550 PMC: 8797919. DOI: 10.21037/tcr-21-37.

References

Amir G, Ramamoorthy C, Riemer R, Reddy V, Hanley F . Neonatal brain protection and deep hypothermic circulatory arrest: pathophysiology of ischemic neuronal injury and protective strategies. Ann Thorac Surg. 2005; 80(5):1955-64. DOI: 10.1016/j.athoracsur.2004.12.040. View

Hayakawa K, Esposito E, Wang X, Terasaki Y, Liu Y, Xing C . Transfer of mitochondria from astrocytes to neurons after stroke. Nature. 2016; 535(7613):551-5. PMC: 4968589. DOI: 10.1038/nature18928. View

Meulmeester E, Melchior F . Cell biology: SUMO. Nature. 2008; 452(7188):709-11. DOI: 10.1038/452709a. View

Levati A, Tommasino C, Moretti M, Paino R, DAliberti G, Santoro F . Giant intracranial aneurysms treated with deep hypothermia and circulatory arrest. J Neurosurg Anesthesiol. 2007; 19(1):25-30. DOI: 10.1097/01.ana.0000211022.96054.4d. View

Saugstad J . MicroRNAs as effectors of brain function with roles in ischemia and injury, neuroprotection, and neurodegeneration. J Cereb Blood Flow Metab. 2010; 30(9):1564-76. PMC: 2932764. DOI: 10.1038/jcbfm.2010.101. View

Loftus L, Gala R, Yang T, Jessick V, Ashley M, Ordonez A . Sumo-2/3-ylation following in vitro modeled ischemia is reduced in delayed ischemic tolerance. Brain Res. 2009; 1272:71-80. PMC: 2774733. DOI: 10.1016/j.brainres.2009.03.034. View

Lee Y, Miyake S, Wakita H, McMullen D, Azuma Y, Auh S . Protein SUMOylation is massively increased in hibernation torpor and is critical for the cytoprotection provided by ischemic preconditioning and hypothermia in SHSY5Y cells. J Cereb Blood Flow Metab. 2006; 27(5):950-62. PMC: 2396349. DOI: 10.1038/sj.jcbfm.9600395. View

Stier G, Verde E . The postoperative care of adult patients exposed to deep hypothermic circulatory arrest. Semin Cardiothorac Vasc Anesth. 2007; 11(1):77-85. DOI: 10.1177/1089253206298010. View

Madathil S, Nelson P, Saatman K, Wilfred B . MicroRNAs in CNS injury: potential roles and therapeutic implications. Bioessays. 2010; 33(1):21-6. PMC: 3033010. DOI: 10.1002/bies.201000069. View

10.

Allen J, Weiss E, Wilson M, Arnaoutakis G, Blue M, Talbot Jr C . Hawley H. Seiler Resident Award. Transcriptional profile of brain injury in hypothermic circulatory arrest and cardiopulmonary bypass. Ann Thorac Surg. 2010; 89(6):1965-71. PMC: 3031914. DOI: 10.1016/j.athoracsur.2010.02.051. View

11.

Martino F, Lorenzen J, Schmidt J, Schmidt M, Broll M, Gorzig Y . Circulating microRNAs are not eliminated by hemodialysis. PLoS One. 2012; 7(6):e38269. PMC: 3371001. DOI: 10.1371/journal.pone.0038269. View

12.

Yang W, Ma Q, Mackensen G, Paschen W . Deep hypothermia markedly activates the small ubiquitin-like modifier conjugation pathway; implications for the fate of cells exposed to transient deep hypothermic cardiopulmonary bypass. J Cereb Blood Flow Metab. 2009; 29(5):886-90. DOI: 10.1038/jcbfm.2009.16. View

13.

Bernard S, Gray T, Buist M, Jones B, Silvester W, Gutteridge G . Treatment of comatose survivors of out-of-hospital cardiac arrest with induced hypothermia. N Engl J Med. 2002; 346(8):557-63. DOI: 10.1056/NEJMoa003289. View

14.

Wang X, Ji B, Yang B, Liu G, Miao N, Yang J . Real-time continuous neuromonitoring combines transcranial cerebral Doppler with near-infrared spectroscopy cerebral oxygen saturation during total aortic arch replacement procedure: a pilot study. ASAIO J. 2012; 58(2):122-6. DOI: 10.1097/MAT.0b013e318241abd3. View

15.

Wilkinson K, Henley J . Mechanisms, regulation and consequences of protein SUMOylation. Biochem J. 2010; 428(2):133-45. PMC: 3310159. DOI: 10.1042/BJ20100158. View

16.

Datwyler A, Lattig-Tunnemann G, Yang W, Paschen W, Lee S, Dirnagl U . SUMO2/3 conjugation is an endogenous neuroprotective mechanism. J Cereb Blood Flow Metab. 2011; 31(11):2152-9. PMC: 3210338. DOI: 10.1038/jcbfm.2011.112. View

17.

Reith J, Jorgensen H, Pedersen P, Nakayama H, Raaschou H, Jeppesen L . Body temperature in acute stroke: relation to stroke severity, infarct size, mortality, and outcome. Lancet. 1996; 347(8999):422-5. DOI: 10.1016/s0140-6736(96)90008-2. View

18.

Lee Y, Castri P, Bembry J, Maric D, Auh S, Hallenbeck J . SUMOylation participates in induction of ischemic tolerance. J Neurochem. 2009; 109(1):257-67. PMC: 2692380. DOI: 10.1111/j.1471-4159.2009.05957.x. View

19.

Fiedler J, Thum T . MicroRNAs looping around angiogenesis. Arterioscler Thromb Vasc Biol. 2011; 31(11):2367-8. DOI: 10.1161/ATVBAHA.111.237602. View