Opposing Kinesin Complexes Queue at Plus Tips to Ensure Microtubule Catastrophe at Cell Ends

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2018 Sep 13

PMID 30206188

Citations 5

Authors

John C Meadows

Liam J Messin

Anton Kamnev

Theresa C Lancaster

Mohan K Balasubramanian

Robert A Cross

Jonathan Ba Millar

Affiliations

Soon will be listed here.

Abstract

In fission yeast, the lengths of interphase microtubule (iMT) arrays are adapted to cell length to maintain cell polarity and to help centre the nucleus and cell division ring. Here, we show that length regulation of iMTs is dictated by spatially regulated competition between MT-stabilising Tea2/Tip1/Mal3 (Kinesin-7) and MT-destabilising Klp5/Klp6/Mcp1 (Kinesin-8) complexes at iMT plus ends. During MT growth, the Tea2/Tip1/Mal3 complex remains bound to the plus ends of iMT bundles, thereby restricting access to the plus ends by Klp5/Klp6/Mcp1, which accumulate behind it. At cell ends, Klp5/Klp6/Mcp1 invades the space occupied by the Tea2/Tip1/Tea1 kinesin complex triggering its displacement from iMT plus ends and MT catastrophe. These data show that , whilst an iMT length-dependent model for catastrophe factor accumulation has validity, length control of iMTs is an emergent property reflecting spatially regulated competition between distinct kinesin complexes at the MT plus tip.

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