Fabrication and Characterization of an Electrospun PHA/Graphene Silver Nanocomposite Scaffold for Antibacterial Applications

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2018 Sep 12

PMID 30201852

Citations 18

Authors

Abdul Mukheem

Kasturi Muthoosamy

Sivakumar Manickam

Kumar Sudesh

Syed Shahabuddin

Rahman Saidur

Noor Akbar

Nanthini Sridewi

Affiliations

Soon will be listed here.

Abstract

Many wounds are unresponsive to currently available treatment techniques and therefore there is an immense need to explore suitable materials, including biomaterials, which could be considered as the crucial factor to accelerate the healing cascade. In this study, we fabricated polyhydroxyalkanoate-based antibacterial mats via an electrospinning technique. One-pot green synthesized graphene-decorated silver nanoparticles (GAg) were incorporated into the fibres of poly-3 hydroxybutarate-co-12 mol.% hydroxyhexanoate (P3HB-co-12 mol.% HHx), a co-polymer of the polyhydroxyalkanoate (PHA) family which is highly biocompatible, biodegradable, and flexible in nature. The synthesized PHA/GAg biomaterial has been characterized by field emission scanning electron microscopy (FESEM), elemental mapping, thermogravimetric analysis (TGA), UV-visible spectroscopy (UV-vis), and Fourier transform infrared spectroscopy (FTIR). An in vitro antibacterial analysis was performed to investigate the efficacy of PHA/GAg against gram-positive () strain 12,600 ATCC and gram-negative () strain 8739 ATCC. The results indicated that the PHA/GAg demonstrated significant reduction of and as compared to bare PHA or PHA- reduced graphene oxide (rGO) in 2 h of time. The value ( < 0.05) was obtained by using a two-sample -test distribution.

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References

Wei L, Lu J, Xu H, Patel A, Chen Z, Chen G . Silver nanoparticles: synthesis, properties, and therapeutic applications. Drug Discov Today. 2014; 20(5):595-601. PMC: 4433816. DOI: 10.1016/j.drudis.2014.11.014. View

Jung W, Koo H, Kim K, Shin S, Kim S, Park Y . Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Appl Environ Microbiol. 2008; 74(7):2171-8. PMC: 2292600. DOI: 10.1128/AEM.02001-07. View

Suk J, Lee W, Lee J, Chou H, Piner R, Hao Y . Enhancement of the electrical properties of graphene grown by chemical vapor deposition via controlling the effects of polymer residue. Nano Lett. 2013; 13(4):1462-7. DOI: 10.1021/nl304420b. View

Lu X, Ciraolo E, Stefenia R, Chen G, Zhang Y, Hirsch E . Sustained release of PI3K inhibitor from PHA nanoparticles and in vitro growth inhibition of cancer cell lines. Appl Microbiol Biotechnol. 2011; 89(5):1423-33. DOI: 10.1007/s00253-011-3101-1. View

Novoselov K, Geim A, Morozov S, Jiang D, Zhang Y, Dubonos S . Electric field effect in atomically thin carbon films. Science. 2004; 306(5696):666-9. DOI: 10.1126/science.1102896. View

Karunamuni R, Naha P, Lau K, Al-Zaki A, Popov A, Delikatny E . Development of silica-encapsulated silver nanoparticles as contrast agents intended for dual-energy mammography. Eur Radiol. 2016; 26(9):3301-9. PMC: 4974128. DOI: 10.1007/s00330-015-4152-y. View

Akhavan O, Ghaderi E . Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano. 2010; 4(10):5731-6. DOI: 10.1021/nn101390x. View

Beyth N, Houri-Haddad Y, Domb A, Khan W, Hazan R . Alternative antimicrobial approach: nano-antimicrobial materials. Evid Based Complement Alternat Med. 2015; 2015:246012. PMC: 4378595. DOI: 10.1155/2015/246012. View

Lee C, Wei X, Kysar J, Hone J . Measurement of the elastic properties and intrinsic strength of monolayer graphene. Science. 2008; 321(5887):385-8. DOI: 10.1126/science.1157996. View

10.

Chen Y, Prasad K, Wang X, Pang H, Yan R, Than A . Enzymeless multi-sugar fuel cells with high power output based on 3D graphene-Co3O4 hybrid electrodes. Phys Chem Chem Phys. 2013; 15(23):9170-6. DOI: 10.1039/c3cp51410b. View

11.

Choi B, Park H, Park T, Yang M, Kim J, Jang S . Solution chemistry of self-assembled graphene nanohybrids for high-performance flexible biosensors. ACS Nano. 2010; 4(5):2910-8. DOI: 10.1021/nn100145x. View

12.

Unnithan A, Gnanasekaran G, Sathishkumar Y, Lee Y, Sang Kim C . Electrospun antibacterial polyurethane-cellulose acetate-zein composite mats for wound dressing. Carbohydr Polym. 2014; 102:884-92. DOI: 10.1016/j.carbpol.2013.10.070. View

13.

Han J, Gurunathan S, Jeong J, Choi Y, Kwon D, Park J . Oxidative stress mediated cytotoxicity of biologically synthesized silver nanoparticles in human lung epithelial adenocarcinoma cell line. Nanoscale Res Lett. 2014; 9(1):459. PMC: 4167841. DOI: 10.1186/1556-276X-9-459. View

14.

Liu S, Zeng T, Hofmann M, Burcombe E, Wei J, Jiang R . Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. ACS Nano. 2011; 5(9):6971-80. DOI: 10.1021/nn202451x. View

15.

Misra S, Ansari T, Valappil S, Mohn D, Philip S, Stark W . Poly(3-hydroxybutyrate) multifunctional composite scaffolds for tissue engineering applications. Biomaterials. 2010; 31(10):2806-15. DOI: 10.1016/j.biomaterials.2009.12.045. View

16.

Muthoosamy K, Bai R, Manickam S . Graphene and graphene oxide as a docking station for modern drug delivery system. Curr Drug Deliv. 2014; 11(6):701-18. DOI: 10.2174/1567201811666140605151600. View

17.

Jayakumar R, Prabaharan M, Sudheesh Kumar P, Nair S, Tamura H . Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv. 2011; 29(3):322-37. DOI: 10.1016/j.biotechadv.2011.01.005. View

18.

Guo H, Wang X, Qian Q, Wang F, Xia X . A green approach to the synthesis of graphene nanosheets. ACS Nano. 2009; 3(9):2653-9. DOI: 10.1021/nn900227d. View

19.

Morones J, Elechiguerra J, Camacho A, Holt K, Kouri J, Tapia Ramirez J . The bactericidal effect of silver nanoparticles. Nanotechnology. 2010; 16(10):2346-53. DOI: 10.1088/0957-4484/16/10/059. View

20.

Shamala T, Divyashree M, Davis R, Kumari K, Vijayendra S, Raj B . Production and characterization of bacterial polyhydroxyalkanoate copolymers and evaluation of their blends by fourier transform infrared spectroscopy and scanning electron microscopy. Indian J Microbiol. 2012; 49(3):251-8. PMC: 3450024. DOI: 10.1007/s12088-009-0031-z. View