T Lymphocyte Cloning from Rejected Human Kidney Allografts. Growth Frequency and Functional/phenotypic Analysis

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1986 Oct 1

PMID 3020092

Citations 7

Authors

J F Moreau

M Bonneville

M A Peyrat

A Godard

Y Jacques

C Desgranges

J P Soulillou

Affiliations

Soon will be listed here.

Abstract

Mechanically harvested lymphocytes invading an irreversibly rejected human kidney allograft were seeded at limiting dilution to calculate the frequency of growing precursors. Optimal growth frequency (1/13) was obtained when Epstein-Barr virus (EBV)-transformed donor B lymphocytes were used as stimulators (D-BLCL) in the presence of interleukin 2 (IL-2). The 55 clones analyzed were all T11+ and T3+, and all expressed DR antigens (45% were T8+ and 55% T4+). Only one clone had a double-labeled (T4+ T8+) surface. All cells proliferated significantly against D-BLCL, although T4+ clones had a significantly shorter average doubling time than T8+ clones. Nearly all T8+ clones were specifically cytotoxic for D-BLCL, while both T4 and T8 did not react against K562, autologous EBV-BLCL, and third-party EBV-BLCL. Detectable IL-2 was found in the culture supernatants of only a minority of clones (all T4+).

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