MiRNA-16 Inhibited Oral Squamous Carcinoma Tumor Growth in Vitro and in Vivo Via Suppressing Wnt/β-catenin Signaling Pathway

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2018 Sep 11

PMID 30197522

Citations 6

Authors

Lijun Liu

Han Jiang

Jin Zhao

Hao Wen

Affiliations

Soon will be listed here.

Abstract

Background: Oral carcinoma, one of the most commonly diagnosed cancers, has a poor prognosis and low survival rate with treatment. In recent years, some studies reported the upregulation of miRNA-16 (miR-16) in the oral carcinoma, whereas some other studies confirmed the downregulation of miR-16. In the current study, we aimed to investigate the function of miR-16 in oral carcinoma.

Materials And Methods: Cell proliferation assay was measured by MTT assay, quantitative real time polymerase chain reaction (qRT-PCR) was used to evaluate the expression of miR-16, and apoptosis was analyzed by flow cytometry. In addition, the expression of proteins was detected by Western blot. Moreover, xenograft tumor model was established to detect the effect of miR-16 in vivo.

Results: The results suggested that miR-16 was downregulated in the oral carcinoma tissues. Overexpression of miR-16 inhibited the growth and proliferation of oral squamous carcinoma cells (OSCCs) and induced apoptosis both in vitro and in vivo, which is due to the suppression of Wnt/β-catenin signaling pathway.

Conclusion: This study provides evidence that overexpression of miR-16 inhibits OSCC growth by regulating Wnt/β-catenin signaling. Our findings suggest that overexpression of miR-16 could be a potential approach for gene therapy of OSCC in future.

Citing Articles

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Integrated analysis of gene expressions and targeted mirnas for explaining crosstalk between oral and esophageal squamous cell carcinomas through an interpretable machine learning approach.

Yadav K, Hasija Y Med Biol Eng Comput. 2024; 63(2):483-495.

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PMID: 34164962 PMC: 8236341. DOI: 10.3349/ymj.2021.62.7.640.

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