: Defining the Role of Efflux Pumps in Resistance to Antimicrobial Therapy, Surface Motility, and Biofilm Formation

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2018 Sep 7

PMID 30186249

Citations 20

Authors

Daniel B Knight

Susan D Rudin

Robert A Bonomo

Philip N Rather

Affiliations

Soon will be listed here.

Abstract

is a member of the (ACB) complex. Increasingly, reports are emerging of the pathogenic profile and multidrug resistance (MDR) phenotype of this species. To define novel therapies to overcome resistance, we queried the role of the major efflux pumps in strain M2 on antimicrobial susceptibility profiles. strains with the following mutations were engineered by allelic replacement; Δ, Δ, and Δ. In these isogenic strains, we show that the Δ mutation increased susceptibility to beta-lactams, beta-lactam/beta-lactamase inhibitors, chloramphenicol, monobactam, tigecycline, and trimethoprim. The Δ mutation had a minor effect on resistance to certain beta-lactams, rifampicin and tigecycline. In addition, the Δ mutation resulted in a significant decrease in surface motility and a minor decrease in biofilm formation. Our results indicate that the efflux pump, AdeIJK, has additional roles outside of antibiotic resistance in .

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