Isobavachalcone Sensitizes Cells to E2-induced Paclitaxel Resistance by Down-regulating CD44 Expression in ER+ Breast Cancer Cells

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2018 Sep 5

PMID 30179299

Citations 11

Authors

Junfeng Shi

Yi Chen

Wenxing Chen

Cuiju Tang

Honghong Zhang

Yuetong Chen

Xiuwei Yang

Zhi Xu

Jingsun Wei

Jinfei Chen

Affiliations

Soon will be listed here.

Abstract

Oestrogen receptor (ER) is expressed in approximately 60%-70% of human breast cancer. Clinical trials and retrospective analyses have shown that ER-positive (ER+) tumours are more tolerant to chemotherapeutic drug resistance than ER-negative (ER-) tumours. In addition, isobavachalcone (IBC) is known as a kind of phytoestrogen with antitumour effect. However, the underlying mechanism of IBC in ER+ breast cancer needs to be elucidated further. Our in vitro experiments showed that IBC could attenuate 17β-estradiol (E )-induced paclitaxel resistance and that E could stimulate CD44 expression in ER+ breast cancer cells but not in ER- cells. Meanwhile, E could promote ERα expression to render ER+ breast cancer cells resistant to paclitaxel. Furthermore, we established paclitaxel-resistant breast cancer cell lines and determined the function of ERα in the enhancement of paclitaxel resistance via the regulation of CD44 transcription. IBC down-regulated ERα and CD44 expression and thus inhibited tumour growth in paclitaxel-resistant xenograft models. Overall, our data demonstrated for the first time that IBC could decrease CD44 expression level via the ERα pathway and make ER+ breast cancer cells sensitive to paclitaxel treatment.

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