Extracts of Protect Astrocytic Cells Under Oxidative Stress With Rotenone

Overview

Journal Front Chem

Specialty Chemistry

Date 2018 Sep 4

PMID 30175092

Citations 9

Authors

Natalia Areiza-Mazo

Jorge Robles

Jairo A Zamudio-Rodriguez

Lisandro Giraldez

Valentina Echeverria

Biviana Barrera-Bailon

Gjumrakch Aliev

Amirhossein Sahebkar

Ghulam Md Ashraf

George E Barreto

Affiliations

Soon will be listed here.

Abstract

The use of medicinal plants to counteract the oxidative damage in neurodegenerative diseases has steadily increased over the last few years. However, the rationale for using these natural compounds and their therapeutic benefit are not well explored. In this study, we evaluated the effect of different extracts on astrocytic cells (T98G) subjected to oxidative damage induced by rotenone. Extracts of fresh and dehydrated fruits of the plant with different polarities were prepared and tested . Our results demonstrated that the ethanolic extract of fresh fruits (EF) and acetone-dehydrated fruit extract (AD) increased cell viability, reduced the formation of reactive oxygen species (ROS) and preserved mitochondrial membrane potential. In contrast, we observed a significant reduction in mitochondrial mass when rotenone-treated cells were co-treated with EF and AD. These effects were accompanied by a reduction in the percentage of cells with fragmented/condensed nuclei and increased expression of endogenous antioxidant defense survival proteins such as ERK1/2. In conclusion, our findings suggest that ethanolic and acetone extracts from are potential medicinal plant extracts to overcome oxidative damage induced by neurotoxic compounds.

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