Comparison of CD9 & CD146 Markers in Endometrial Stromal Cells of Fertile & Infertile Females

Overview

Journal Indian J Med Res

Specialty General Medicine

Date 2018 Sep 1

PMID 30168486

Citations 2

Authors

Manisha Sudhir Chaudhari-Kank

Kusum Zaveri

Vistasp Antia

Indira Hinduja

Affiliations

Soon will be listed here.

Abstract

Background & Objectives: CD9 and CD146 are important adhesion molecules that play a role in the implantation of an embryo. This study was undertaken to correlate the expression of these markers in fertile and infertile women's endometrial stromal cells.

Methods: Human endometrial stromal cell culture from endometrial biopsies of fertile (n=50) and infertile females (n=50) was performed and primary cell lines were established. Expression of CD9 and CD146 was studied for all the 100 cell lines with the help of flow cytometry. Gene expression of CD9 and CD146 was performed by real-time polymerase chain reaction.

Results: There was a significant difference in endometrial stromal cells of fertile and infertile females. Flow cytometric results revealed significantly lower expression of CD9 (P=0.0126) and CD146 (P=0.0006) in the infertile endometrial stromal cells as compared to fertile endometrial stromal cells. These results were comparable with real-time data.

Interpretation & Conclusions: This study showed that endometrial stromal cells from infertile females had lower expression of adhesion molecules, CD9 and CD146. Our findings suggest that CD9 and CD146 may have a role in infertility. Infertile female's endometrial stromal cells have decreased expression of CD9 and CD146 which can be the cause of infertility related to implantation failure.

Citing Articles

Melanoma Cell Adhesion Molecule (CD 146) in Endometrial Physiology and Disorder.

Hilage P, Damle M, Sharma R, Joshi M Adv Exp Med Biol. 2024; 1474():131-148.

PMID: 39400880 DOI: 10.1007/5584_2024_826.

A Comparative Study of Gene Expression in Menstrual Blood-Derived Stromal Cells between Endometriosis and Healthy Women.

Sahraei S, Davoodi Asl F, Kalhor N, Sheykhhasan M, Fazaeli H, Soleymani Moud S Biomed Res Int. 2022; 2022:7053521.

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Role of CD146 (MCAM) in Physiological and Pathological Angiogenesis-Contribution of New Antibodies for Therapy.

Joshkon A, Heim X, Dubrou C, Bachelier R, Traboulsi W, Stalin J Biomedicines. 2020; 8(12).

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