Human Cytomegalovirus-infected Cells Release Extracellular Vesicles That Carry Viral Surface Proteins

Overview

Journal Virology

Specialty Microbiology

Date 2018 Aug 31

PMID 30165311

Citations 30

Authors

Sonia Zicari

Anush Arakelyan

Rogers Alberto Nahui Palomino

Wendy Fitzgerald

Christophe Vanpouille

Anna Lebedeva

Alain Schmitt

Morgane Bomsel

William Britt

Leonid Margolis

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) released by virus-infected cells typically incorporate host and viral components inside the vesicles (cargo molecules). Here, we investigated if human cytomegalovirus (HCMV) proteins are incorporated in EV outer membrane released by HCMV-infected cells. We separated EVs from HCMV using an iodixanol step-gradient and found that the separated vesicles carried EV markers such as the tetraspanin CD63 and Rab27A. Flow analysis of individual EVs demonstrated that on average, 15 ± 3.7% of EVs were positive for gB, 5.3 ± 2.3% were positive for gH and 3.74 ± 1.5% were positive for both gB and gH. In light of previous findings demonstrating HIV envelope proteins in EV membranes, the presence of viral protein at the surface of EVs released by HCMV-infected cells indicated that viral membrane proteins incorporated in EVs released by virus-infected cells may be a general phenomenon.

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