Large T Antigens of Simian Virus 40 and Polyomavirus Efficiently Establish Primary Fibroblasts

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1986 Sep 1

PMID 3016337

Citations 47

Authors

P S Jat

P A Sharp

Affiliations

Soon will be listed here.

Abstract

Recombinant retroviruses that transduce the simian virus 40 (SV40) large T antigen or the polyomavirus large T antigen as well as encoding resistance to antibiotic G418 were used to investigate whether these genes alone were sufficient for immortalization of primary cells. The results provided definitive evidence that either viral gene can efficiently establish primary fibroblasts. The capability of the SV40 large T antigen to establish primary fibroblasts was undiminished by a mutation that alters its binding to sequences within the origin of replication. Surprisingly, most of the primary cells established by the expression of the SV40 large T antigen did not have a transformed phenotype. This suggests that transformation by SV40 is not simply due to a high level of expression of the SV40 large T antigen and stabilization of cellular p53.

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