Detection of Microcystin-producing Cyanobacteria in Water Samples Using Loop-mediated Isothermal Amplification Targeting Gene

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2018 Aug 28

PMID 30148028

Citations 2

Authors

Mohandass Ramya

Muthukrishnan Kayalvizhi

Gopalakrishnan Haripriya

Pasupathi Rathinasabapathi

Affiliations

Soon will be listed here.

Abstract

Microcystin toxin-producing cyanobacteria are known to have harmful effects on humans and animals. We have developed a loop-mediated isothermal amplification (LAMP)-based detection method by targeting the microcystin synthetase B gene (), the gene responsible for the production of microcystin. The sensitivity of the method was found to be 1 fg per reaction, and it was 1000-fold higher than the conventional PCR. The LAMP method was able to amplify the target gene with a minimum amount of dNTP (0.4 mM), which further reduces the cost of reaction. The improved LAMP assay could detect the presence of the toxin-producing cyanobacteria in water samples within 2 h of time, which demonstrates the rapidness of the method. Freshwater samples were screened using the developed LAMP, and seven water samples collected from lakes and a bird sanctuary tested positive for gene harboring cyanobacteria, and negative in all other drinking waters. Hence, the developed LAMP could be a possible alternative to the existing molecular methods for screening for microcystin in environmental samples with greater sensitivity.

Citing Articles

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