Lack of Subclass Selection During Density Purification of Human Lymphocytes

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1977 May 1

PMID 301453

Citations 4

Authors

P Hayry

T H Totterman

A Ranki

Affiliations

Soon will be listed here.

Abstract

The percentage of acid alpha-naphthyl acetate esterase (ANAE) marker-carrying lymphocytes (T-lymphocytes) was determined directly from the blood, after density purification of lymphocytes over Ficoll-Isopaque and removal of monocytes by iron powder plus magnet. The effect of Ficoll-Isopaque centrifugation was insignificant; neither did the iron powder plus magnet procedure increase the relative proportion of the T cells in these preparations. We therefore conclude that in optimal conditions there is no subclass-specific enrichment of T or B lymphocytes during the purification procedure.

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References

Ranki A, Totterman T, Hayry P . Identification of resting human T and B lymphocytes by acid alpha-naphthyl acetate esterase staining combined with rosette formation with Staphylococcus aureus strain Cowan 1. Scand J Immunol. 1976; 5(10):1129-38. DOI: 10.1111/j.1365-3083.1976.tb00254.x. View

Ranki A, Totterman T, Hayry P . Identification of mouse T and B lymphocytes from cytocentrifuged cell smears. Clin Exp Immunol. 1976; 26(3):632-40. PMC: 1540959. View

Brown G, Greaves M . Cell surface markers for human T and B lymphocytes. Eur J Immunol. 1974; 4(4):302-10. DOI: 10.1002/eji.1830040414. View

Jondal M, Holm G, Wigzell H . Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2):207-15. PMC: 2139198. DOI: 10.1084/jem.136.2.207. View

Dickler H, KUNKEL H . Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972; 136(1):191-6. PMC: 2139185. DOI: 10.1084/jem.136.1.191. View