Loss of Gαq Impairs Regulatory B-cell Function

Overview

Journal Arthritis Res Ther

Publisher Biomed Central

Specialty Rheumatology

Date 2018 Aug 26

PMID 30143054

Citations 2

Authors

Yan He

Xiaoqing Yuan

Yan Li

Chunlian Zhong

Yuan Liu

Hongyan Qian

Jingxiu Xuan

Lihua Duan

Guixiu Shi

Affiliations

Soon will be listed here.

Abstract

Background: Recent studies have shown a crucial role of Gαq in immune regulation, but how Gαq modulates regulatory B-cell (Breg) function is still unclear. We address this here.

Methods: CD19IL-10 Bregs of wild-type (WT) and Gnaq mice were analyzed by flow cytometry after stimulation by lipopolysaccharide. The WT and Gnaq Bregs were isolated and cocultured with WT CD4CD25 T cells in the presence of T-activator, and the proliferation of T cells and differentiation of regulatory T cells (Tregs) were analyzed by flow cytometry. We used inhibitors of PI3 kinase (PI3K), extracellular regulated protein kinases 1/2 (Erk1/2), and p38 mitogen-activated protein kinase (p38 MAPK) to detect the pathways involved in the regulation of Gαq on Breg differentiation, which were confirmed by western blot analysis. Furthermore, the expression level of Gαq was assessed by quantitative real-time PCR in peripheral blood mononuclear cells (PBMCs) from healthy controls and rheumatoid arthritis patients. The frequency of CD19CD24CD38 B cells in PBMCs was detected by flow cytometry, and the association of the Gαq mRNA expression level and the frequency of CD19CD24CD38 B cells was analyzed by Spearman test.

Results: The differentiation of CD19IL-10 Bregs was inhibited in the Gnaq mice. In addition, Gαq depletion showed an impaired suppressive function of Bregs on T-cell proliferation, which might be due to the decreased Treg expansion. Mechanically, our data demonstrated that the PI3K, Erk1/2, and p38 MAPK signaling pathways were required for regulation of Gαq on Bregs, and blockage of these signaling pathways impaired Breg differentiation. Consistent with our previous studies, we also found a decreased frequency of CD19CD24CD38 Bregs in rheumatoid arthritis patients. As expected, a significantly positive correlation was investigated between CD19CD24CD38 Bregs with Gαq mRNA expression.

Conclusions: Our results indicate that Gαq plays a critical role in the differentiation and immunosuppression of Bregs, and it may provide a new therapeutic target for autoimmune diseases.

Citing Articles

Heterozygous Gnaq deficiency enhances Ifi202b/IFI16 and NF-κB activation in endothelial cells and exacerbates lupus nephritis pathology.

Zhang L, He Y, Zhang M, Zhang J, Zhuang W, Sun Y iScience. 2024; 27(8):110350.

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G Protein-Coupled receptors and heterotrimeric G proteins as cancer drivers.

Arang N, Gutkind J FEBS Lett. 2020; 594(24):4201-4232.

PMID: 33270228 PMC: 8849590. DOI: 10.1002/1873-3468.14017.

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