Circular RNA CEP128 Acts As a Sponge of MiR-145-5p in Promoting the Bladder Cancer Progression Via Regulating SOX11

Overview

Journal Mol Med

Publisher Biomed Central

Specialties General Medicine
Molecular Biology

Date 2018 Aug 24

PMID 30134837

Citations 53

Authors

Zhun Wu

Wei Huang

Xuegang Wang

Tao Wang

Yuedong Chen

Bin Chen

Rongfu Liu

Peide Bai

Jinchun Xing

Affiliations

Soon will be listed here.

Abstract

Background: This study aimed to investigate the effect of over-expressing circular RNA CEP128 (circCEP128) on cell functions and explore the molecular mechanism of which in bladder carcinoma.

Methods: The differentially expressed circRNAs and mRNAs in bladder carcinoma cells and cells in adjacent tissues were screened out using microarray analysis. Expression levels of circRNAs and mRNAs in tissues and cells were determined by qRT-PCR. Expression of SOX11 was detected by western blot. Luciferase reporter assay and RNA pull-down assay were used to investigate the interactions between the specific circRNA, miRNA and mRNA. Cell cycle and apoptosis were measured using flow cytometry after transfection. MTT assay was also performed to detect the cell proliferation.

Results: In present study, circCEP128 and SOX11 were observed significantly up-regulated in bladder cancer tissues, while the expression of miR-145-5p was decreased in cancer samples compared to normal samples. Cytoscape was used to visualize circCEP128-miRNA-target gene interactions based on the TargetScan and circular RNA interactome, which revealed that circCEP128 served as a sponge of miR-145-5p and indirectly regulated SOX11. Knockdown of circCEP128 induced the inhibition of cell proliferation and the increased bladder cancer cell apoptosis rate.

Conclusions: CircCEP128 functions as a ceRNA for miR-145-5p, which could up regulates SOX11 and further promotes cell proliferation and inhibits cell apoptosis of bladder cancer.

Citing Articles

Bladder cancer: non-coding RNAs and exosomal non-coding RNAs.

Zhao J, Ma Y, Zheng X, Sun Z, Lin H, Du C Funct Integr Genomics. 2024; 24(5):147.

PMID: 39217254 DOI: 10.1007/s10142-024-01433-9.

A Combination of Microarray-Based Profiling and Biocomputational Analysis Identified miR331-3p and hsa-let-7d-5p as Potential Biomarkers of Ulcerative Colitis Progression to Colorectal Cancer.

Chacon-Millan P, Lama S, Del Gaudio N, Gravina A, Federico A, Pellegrino R Int J Mol Sci. 2024; 25(11).

PMID: 38891888 PMC: 11171846. DOI: 10.3390/ijms25115699.

Construction of CDKN2A-related competitive endogenous RNA network and identification of GAS5 as a prognostic indicator for hepatocellular carcinoma.

Pan Y, Zhang Y, Wang L, Wu L, Ma Y, Fang Z World J Gastrointest Oncol. 2024; 16(4):1514-1531.

PMID: 38660664 PMC: 11037068. DOI: 10.4251/wjgo.v16.i4.1514.

Retraction Note: Silencing of Histone Deacetylase 9 Expression in Podocytes Attenuates Kidney Injury in Diabetic Nephropathy.

Liu F, Zong M, Wen X, Li X, Wang J, Wang Y Sci Rep. 2024; 14(1):7734.

PMID: 38565577 PMC: 10987519. DOI: 10.1038/s41598-024-58326-x.

Dysregulation and antimetastatic function of circLRIG1 modulated by miR-214-3p/LRIG1 axis in bladder carcinoma.

Cheng S, Li C, Liu L, Liu X, Li M, Zhuo J Biol Direct. 2024; 19(1):20.

PMID: 38454507 PMC: 10918934. DOI: 10.1186/s13062-023-00446-x.

References

Minami K, Taniguchi K, Sugito N, Kuranaga Y, Inamoto T, Takahara K . MiR-145 negatively regulates Warburg effect by silencing KLF4 and PTBP1 in bladder cancer cells. Oncotarget. 2017; 8(20):33064-33077. PMC: 5464850. DOI: 10.18632/oncotarget.16524. View

Zhong Z, Huang M, Lv M, He Y, Duan C, Zhang L . Circular RNA MYLK as a competing endogenous RNA promotes bladder cancer progression through modulating VEGFA/VEGFR2 signaling pathway. Cancer Lett. 2017; 403:305-317. DOI: 10.1016/j.canlet.2017.06.027. View

Noguchi S, Yamada N, Kumazaki M, Yasui Y, Iwasaki J, Naito S . socs7, a target gene of microRNA-145, regulates interferon-β induction through STAT3 nuclear translocation in bladder cancer cells. Cell Death Dis. 2013; 4:e482. PMC: 3734815. DOI: 10.1038/cddis.2013.11. View

Markopoulos G, Roupakia E, Tokamani M, Chavdoula E, Hatziapostolou M, Polytarchou C . A step-by-step microRNA guide to cancer development and metastasis. Cell Oncol (Dordr). 2017; 40(4):303-339. DOI: 10.1007/s13402-017-0341-9. View

Shang X, Li G, Liu H, Li T, Liu J, Zhao Q . Comprehensive Circular RNA Profiling Reveals That hsa_circ_0005075, a New Circular RNA Biomarker, Is Involved in Hepatocellular Crcinoma Development. Medicine (Baltimore). 2016; 95(22):e3811. PMC: 4900729. DOI: 10.1097/MD.0000000000003811. View

Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A . Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 2013; 495(7441):333-8. DOI: 10.1038/nature11928. View

Fang G, Liu J, Wang Q, Huang X, Yang R, Pang Y . MicroRNA-223-3p Regulates Ovarian Cancer Cell Proliferation and Invasion by Targeting SOX11 Expression. Int J Mol Sci. 2017; 18(6). PMC: 5486031. DOI: 10.3390/ijms18061208. View

Li Y, Zheng F, Xiao X, Xie F, Tao D, Huang C . CircHIPK3 sponges miR-558 to suppress heparanase expression in bladder cancer cells. EMBO Rep. 2017; 18(9):1646-1659. PMC: 5579470. DOI: 10.15252/embr.201643581. View

Huang M, Zhong Z, Lv M, Shu J, Tian Q, Chen J . Comprehensive analysis of differentially expressed profiles of lncRNAs and circRNAs with associated co-expression and ceRNA networks in bladder carcinoma. Oncotarget. 2016; 7(30):47186-47200. PMC: 5216934. DOI: 10.18632/oncotarget.9706. View

10.

Enokida H, Yoshino H, Matsushita R, Nakagawa M . The role of microRNAs in bladder cancer. Investig Clin Urol. 2016; 57 Suppl 1:S60-76. PMC: 4910767. DOI: 10.4111/icu.2016.57.S1.S60. View

11.

Xu H, Guo S, Li W, Yu P . The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells. Sci Rep. 2015; 5:12453. PMC: 4515639. DOI: 10.1038/srep12453. View

12.

Oliemuller E, Kogata N, Bland P, Kriplani D, Daley F, Haider S . SOX11 promotes invasive growth and ductal carcinoma in situ progression. J Pathol. 2017; 243(2):193-207. PMC: 5637904. DOI: 10.1002/path.4939. View

13.

Meggendorfer M, Kern W, Haferlach C, Haferlach T, Schnittger S . SOX11 overexpression is a specific marker for mantle cell lymphoma and correlates with t(11;14) translocation, CCND1 expression and an adverse prognosis. Leukemia. 2013; 27(12):2388-91. DOI: 10.1038/leu.2013.141. View

14.

Zhong Z, Lv M, Chen J . Screening differential circular RNA expression profiles reveals the regulatory role of circTCF25-miR-103a-3p/miR-107-CDK6 pathway in bladder carcinoma. Sci Rep. 2016; 6:30919. PMC: 4971518. DOI: 10.1038/srep30919. View

15.

Hansen T, Jensen T, Clausen B, Bramsen J, Finsen B, Damgaard C . Natural RNA circles function as efficient microRNA sponges. Nature. 2013; 495(7441):384-8. DOI: 10.1038/nature11993. View

16.

Li F, Zhang L, Li W, Deng J, Zheng J, An M . Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/β-catenin pathway. Oncotarget. 2015; 6(8):6001-13. PMC: 4467417. DOI: 10.18632/oncotarget.3469. View

17.

Sernbo S, Gustavsson E, Brennan D, Gallagher W, Rexhepaj E, Rydnert F . The tumour suppressor SOX11 is associated with improved survival among high grade epithelial ovarian cancers and is regulated by reversible promoter methylation. BMC Cancer. 2011; 11:405. PMC: 3187763. DOI: 10.1186/1471-2407-11-405. View