» Articles » PMID: 38454507

Dysregulation and Antimetastatic Function of CircLRIG1 Modulated by MiR-214-3p/LRIG1 Axis in Bladder Carcinoma

Overview
Journal Biol Direct
Publisher Biomed Central
Specialty Biology
Date 2024 Mar 8
PMID 38454507
Authors
Affiliations
Soon will be listed here.
Abstract

CircLRIG1, a newly discovered circRNA, has yet to have its potential function and biological processes reported. This study explored the role of circLRIG1 in the development and progression of bladder carcinoma and its potential molecular mechanisms. Techniques such as qRT-PCR, Western blot, various cellular assays, and in vivo models were used to investigate mRNA and protein levels, cell behavior, molecular interactions, and tumor growth. The results showed that both circLRIG1 and LRIG1 were significantly reduced in bladder carcinoma tissues and cell lines. Low circLRIG1 expression was associated with poor patient prognosis. Overexpressing circLRIG1 inhibited bladder carcinoma cell growth, migration, and invasion, promoted apoptosis, and decreased tumor growth and metastasis in vivo. Importantly, circLRIG1 was found to sponge miR-214-3p, enhancing LRIG1 expression, and its overexpression also modulated protein levels of E-cadherin, N-cadherin, Vimentin, and LRIG1. Similar effects were observed with LRIG1 overexpression. Notably, a positive correlation was found between circLRIG1 and LRIG1 expression in bladder carcinoma tissues. Additionally, the tumor-suppressing effect of circLRIG1 was reversed by overexpressing miR-214-3p or silencing LRIG1. The study concludes that circLRIG1 suppresses bladder carcinoma progression by enhancing LRIG1 expression via sponging miR-214-3p, providing a potential strategy for early diagnosis and treatment of bladder carcinoma.

Citing Articles

Non-coding RNAs in bladder cancer, a bridge between gut microbiota and host?.

Zou J, Xu B, Luo P, Chen T, Duan H Front Immunol. 2024; 15:1482765.

PMID: 39628486 PMC: 11611751. DOI: 10.3389/fimmu.2024.1482765.

References
1.
Ye W, Chen L, Feng C, Liang T . CircMYLK promotes the growth, migration, invasion, and survival of bladder cancer cells by upregulating CCND3 level via competitively binding to miR-34a. Drug Dev Res. 2021; 82(8):1206-1216. DOI: 10.1002/ddr.21835. View

2.
Wu Z, Liu J, Dai R, Wu S . Current status and future perspectives of immunotherapy in bladder cancer treatment. Sci China Life Sci. 2020; 64(4):512-533. DOI: 10.1007/s11427-020-1768-y. View

3.
Lindstrom A, Ekman K, Stendahl U, Tot T, Henriksson R, Hedman H . LRIG1 and squamous epithelial uterine cervical cancer: correlation to prognosis, other tumor markers, sex steroid hormones, and smoking. Int J Gynecol Cancer. 2007; 18(2):312-7. DOI: 10.1111/j.1525-1438.2007.01021.x. View

4.
Liu P, Li X, Guo X, Chen J, Li C, Chen M . Circular RNA DOCK1 promotes bladder carcinoma progression via modulating circDOCK1/hsa-miR-132-3p/Sox5 signalling pathway. Cell Prolif. 2019; 52(4):e12614. PMC: 6668968. DOI: 10.1111/cpr.12614. View

5.
Chen W, Zheng R, Baade P, Zhang S, Zeng H, Bray F . Cancer statistics in China, 2015. CA Cancer J Clin. 2016; 66(2):115-32. DOI: 10.3322/caac.21338. View