LINC00312 Represses Proliferation and Metastasis of Colorectal Cancer Cells by Regulation of MiR-21

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2018 Aug 23

PMID 30134003

Citations 22

Authors

Gang Li

Changming Wang

Yongbing Wang

Bin Xu

Wenzhong Zhang

Affiliations

Soon will be listed here.

Abstract

Long non-coding RNAs (lncRNAs) have emerged as important regulators of cancer, including colorectal cancer (CRC). The exact expression pattern of long intergenic noncoding RNA 00312 (LINC00312) in CRC and its mechanisms of action have not been reported. Here, we found that LINC00312 is underexpressed in CRC tissues and cell lines. Functional experiments suggested that LINC00312 suppresses growth, migration and invasion of CRC cells in vitro and attenuates tumour proliferation and metastasis in vivo. Mechanistically, LINC00312 was found to regulate the malignancy of CRC cells by binding to miR-21 and by functioning as a tumour suppressor targeting PTEN. Overexpression of miR-21 or knockdown of PTEN attenuated the LINC00312-mediated inhibition of CRC cell proliferation and invasion. Taken together, our results elucidate the role of the LINC00312-miR-21-PTEN axis in CRC cell proliferation and tumour progression and may lead to new lncRNA-based diagnostics or therapeutics for CRC.

Citing Articles

Diagnostic role of circulating long non-coding RNA LINC00312 in patients with non-small cell lung cancer: a retrospective study.

Zhang R, Jiang Y, Gu J, Zhang X, Xie Y BMC Cancer. 2025; 25(1):47.

PMID: 39789501 PMC: 11721336. DOI: 10.1186/s12885-024-13393-1.

Transcriptomic analysis reveals regulation of adipogenesis via long non-coding RNA, alternative splicing, and alternative polyadenylation.

Mostafa S, Wang L, Tian B, Graber J, Moore C Sci Rep. 2024; 14(1):16964.

PMID: 39043790 PMC: 11266407. DOI: 10.1038/s41598-024-67648-9.

Role of MicroRNA-21 in Prostate Cancer Progression and Metastasis: Molecular Mechanisms to Therapeutic Targets.

Singh V, Rajak N, Singh Y, Singh A, Giri R, Garg N Ann Surg Oncol. 2024; 31(7):4795-4808.

PMID: 38758485 DOI: 10.1245/s10434-024-15453-z.

microRNA 21 and long non-coding RNAs interplays underlie cancer pathophysiology: A narrative review.

Giordo R, Ahmadi F, Husaini N, Al-Nuaimi N, Ahmad S, Pintus G Noncoding RNA Res. 2024; 9(3):831-852.

PMID: 38586315 PMC: 10995982. DOI: 10.1016/j.ncrna.2024.03.013.

Unraveling the role of disulfidptosis-related LncRNAs in colon cancer: a prognostic indicator for immunotherapy response, chemotherapy sensitivity, and insights into cell death mechanisms.

Chi H, Huang J, Yan Y, Jiang C, Zhang S, Chen H Front Mol Biosci. 2023; 10:1254232.

PMID: 37916187 PMC: 10617599. DOI: 10.3389/fmolb.2023.1254232.

References

Liu K, Huang W, Yan D, Luo Q, Min X . Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p. Biosci Rep. 2017; 37(4). PMC: 5518463. DOI: 10.1042/BSR20170109. View

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M . Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2014; 136(5):E359-86. DOI: 10.1002/ijc.29210. View

Wu Y, Song Y, Xiong Y, Wang X, Xu K, Han B . MicroRNA-21 (Mir-21) Promotes Cell Growth and Invasion by Repressing Tumor Suppressor PTEN in Colorectal Cancer. Cell Physiol Biochem. 2017; 43(3):945-958. DOI: 10.1159/000481648. View

Yoon J, Abdelmohsen K, Gorospe M . Functional interactions among microRNAs and long noncoding RNAs. Semin Cell Dev Biol. 2014; 34:9-14. PMC: 4163095. DOI: 10.1016/j.semcdb.2014.05.015. View

Oue N, Anami K, Schetter A, Moehler M, Okayama H, Khan M . High miR-21 expression from FFPE tissues is associated with poor survival and response to adjuvant chemotherapy in colon cancer. Int J Cancer. 2013; 134(8):1926-34. PMC: 3947446. DOI: 10.1002/ijc.28522. View

Cui B, Li B, Liu Q, Cui Y . lncRNA CCAT1 Promotes Glioma Tumorigenesis by Sponging miR-181b. J Cell Biochem. 2017; 118(12):4548-4557. DOI: 10.1002/jcb.26116. View

Huarte M . The emerging role of lncRNAs in cancer. Nat Med. 2015; 21(11):1253-61. DOI: 10.1038/nm.3981. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Yang G, Lu X, Yuan L . LncRNA: a link between RNA and cancer. Biochim Biophys Acta. 2014; 1839(11):1097-109. DOI: 10.1016/j.bbagrm.2014.08.012. View

10.

Xiong B, Cheng Y, Ma L, Zhang C . MiR-21 regulates biological behavior through the PTEN/PI-3 K/Akt signaling pathway in human colorectal cancer cells. Int J Oncol. 2012; 42(1):219-28. DOI: 10.3892/ijo.2012.1707. View

11.

Yu H, Xu Q, Liu F, Ye X, Wang J, Meng X . Identification and validation of long noncoding RNA biomarkers in human non-small-cell lung carcinomas. J Thorac Oncol. 2015; 10(4):645-54. DOI: 10.1097/JTO.0000000000000470. View

12.

Li G, Wang C, Wang Y, Xu B, Zhang W . LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR-21. J Cell Mol Med. 2018; 22(11):5565-5572. PMC: 6201213. DOI: 10.1111/jcmm.13830. View

13.

Zhu Q, Lv T, Wu Y, Shi X, Liu H, Song Y . Long non-coding RNA 00312 regulated by HOXA5 inhibits tumour proliferation and promotes apoptosis in Non-small cell lung cancer. J Cell Mol Med. 2017; 21(9):2184-2198. PMC: 5571553. DOI: 10.1111/jcmm.13142. View

14.

Wang L, Sun K, Wu D, Xiu Y, Chen X, Chen S . DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR-490-3p and altering CDK1 expression. J Cell Mol Med. 2017; 21(11):3055-3065. PMC: 5661118. DOI: 10.1111/jcmm.13217. View

15.

Kang W, Lee J, Oh S, Lee S, Jung C . Stromal expression of miR-21 in T3-4a colorectal cancer is an independent predictor of early tumor relapse. BMC Gastroenterol. 2015; 15:2. PMC: 4308857. DOI: 10.1186/s12876-015-0227-0. View

16.

Zhang W, Huang C, Gong Z, Zhao Y, Tang K, Li X . Expression of LINC00312, a long intergenic non-coding RNA, is negatively correlated with tumor size but positively correlated with lymph node metastasis in nasopharyngeal carcinoma. J Mol Histol. 2013; 44(5):545-54. DOI: 10.1007/s10735-013-9503-x. View

17.

Siegel R, Miller K, Jemal A . Cancer statistics, 2015. CA Cancer J Clin. 2015; 65(1):5-29. DOI: 10.3322/caac.21254. View

18.

Li C, Zhao L, Chen Y, He T, Chen X, Mao J . MicroRNA-21 promotes proliferation, migration, and invasion of colorectal cancer, and tumor growth associated with down-regulation of sec23a expression. BMC Cancer. 2016; 16:605. PMC: 4974737. DOI: 10.1186/s12885-016-2628-z. View

19.

Wu K, Zhao Z, Liu K, Zhang J, Li G, Wang L . Long noncoding RNA lnc-sox5 modulates CRC tumorigenesis by unbalancing tumor microenvironment. Cell Cycle. 2017; 16(13):1295-1301. PMC: 5531622. DOI: 10.1080/15384101.2017.1317416. View

20.

Chen D, Sun Q, Cheng X, Zhang L, Song W, Zhou D . Genome-wide analysis of long noncoding RNA (lncRNA) expression in colorectal cancer tissues from patients with liver metastasis. Cancer Med. 2016; 5(7):1629-39. PMC: 4867661. DOI: 10.1002/cam4.738. View