Secretion of Heat Shock -60, -70 KD Protein, IL-1β and TNFα Levels in Serum of a Term Normal Pregnancy and Patients with Pre-eclampsia Development

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2018 Aug 23

PMID 30133944

Citations 8

Authors

Maria C Alvarez-Cabrera

Edgar Barrientos-Galeana

Asyadette Barrera-Garcia

Mauricio Osorio-Caballero

Jesus F Acevedo

Oscar Flores-Herrera

Nestor F Diaz

Anayansi Molina-Hernandez

Guadalupe Garcia-Lopez

Hector Flores-Herrera

Affiliations

Soon will be listed here.

Abstract

The extracellular heat shock proteins (eHsp) family act as molecular chaperones regulating folding, transporting protein and are associated with immune modulation in different physiological and pathological processes. They have been localized in different gestational tissues and their concentration in amniotic fluid and serum has been determined. In the present study, we proposed to determine the concentration of eHsp-60, -70, IL-1β and TNFα in the serum of pregnant patients with 34 weeks of gestation with and without clinical evidences of preeclampsia (PE). Our results indicate significant increase of these markers in patients with PE with respect to healthy pregnant patients without active labor. Finally, the concentration of eHsp-60 and -70 correlated positively with the hepatic dysfunction markers uric acid, lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) glutamic pyruvic transaminase (GPT), and inflammatory IL-1β and TNFα response. In conclusion, our results demonstrate a strong associated between Hsp and marker of hepatic dysfunction.

Citing Articles

Deciphering the immunological interactions: targeting preeclampsia with Hydroxychloroquine's biological mechanisms.

Gajic M, Schroder-Heurich B, Mayer-Pickel K Front Pharmacol. 2024; 15:1298928.

PMID: 38375029 PMC: 10875033. DOI: 10.3389/fphar.2024.1298928.

PRM-based quantitative proteomics analysis of altered HSP abundance in villi and decidua of patients with early missed abortion.

Chen X, Chen X, Luo H, Xia L, Huang S, Chen Q Proteome Sci. 2023; 21(1):12.

PMID: 37587463 PMC: 10429090. DOI: 10.1186/s12953-023-00213-w.

First-trimester plasma extracellular heat shock proteins levels and risk of preeclampsia.

Robellada-Zarate C, Luna-Palacios J, Caballero C, Acuna-Gonzalez J, Lara-Pereyra I, Gonzalez-Azpeitia D J Cell Mol Med. 2023; 27(9):1206-1213.

PMID: 37002651 PMC: 10148059. DOI: 10.1111/jcmm.17674.

Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis.

Canul-Euan A, Zuniga-Gonzalez G, Palacios-Luna J, Maida-Claros R, Diaz N, Saltigeral-Tigeral P Front Pediatr. 2021; 9:740274.

PMID: 34900858 PMC: 8660587. DOI: 10.3389/fped.2021.740274.

Heat Shock Proteins and Their Role in Pregnancy: Redefining the Function of "Old Rum in a New Bottle".

Jee B, Dhar R, Singh S, Karmakar S Front Cell Dev Biol. 2021; 9:648463.

PMID: 33996811 PMC: 8116900. DOI: 10.3389/fcell.2021.648463.

References

Abdulsid A, Lyall F . Heat shock protein 27 expression is spatially distributed in human placenta and selectively regulated during preeclampsia. J Reprod Immunol. 2013; 101-102:89-95. DOI: 10.1016/j.jri.2013.09.002. View

Abdulsid A, Hanretty K, Lyall F . Heat shock protein 70 expression is spatially distributed in human placenta and selectively upregulated during labor and preeclampsia. PLoS One. 2013; 8(1):e54540. PMC: 3557260. DOI: 10.1371/journal.pone.0054540. View

Stocki P, Wang X, Dickinson A . Inducible heat shock protein 70 reduces T cell responses and stimulatory capacity of monocyte-derived dendritic cells. J Biol Chem. 2012; 287(15):12387-94. PMC: 3320988. DOI: 10.1074/jbc.M111.307579. View

Epstein Shochet G, Komemi O, Sadeh-Mestechkin D, Pomeranz M, Fishman A, Drucker L . Heat shock protein-27 (HSP27) regulates STAT3 and eIF4G levels in first trimester human placenta. J Mol Histol. 2016; 47(6):555-563. DOI: 10.1007/s10735-016-9699-7. View

Molvarec A, Rigo Jr J, Lazar L, Balogh K, Mako V, Cervenak L . Increased serum heat-shock protein 70 levels reflect systemic inflammation, oxidative stress and hepatocellular injury in preeclampsia. Cell Stress Chaperones. 2008; 14(2):151-9. PMC: 2727991. DOI: 10.1007/s12192-008-0067-8. View

Walker J . Pre-eclampsia. Lancet. 2000; 356(9237):1260-5. DOI: 10.1016/S0140-6736(00)02800-2. View

Fukushima A, Kawahara H, Isurugi C, Syoji T, Oyama R, Sugiyama T . Changes in serum levels of heat shock protein 70 in preterm delivery and pre-eclampsia. J Obstet Gynaecol Res. 2005; 31(1):72-7. DOI: 10.1111/j.1447-0756.2005.00244.x. View

Molvarec A, Rigo Jr J, Nagy B, Walentin S, Szalay J, Fust G . Serum heat shock protein 70 levels are decreased in normal human pregnancy. J Reprod Immunol. 2007; 74(1-2):163-9. DOI: 10.1016/j.jri.2006.12.002. View

Maynard S, Crawford S, Bathgate S, Yan J, Robidoux L, Moore M . Gestational angiogenic biomarker patterns in high risk preeclampsia groups. Am J Obstet Gynecol. 2013; 209(1):53.e1-9. DOI: 10.1016/j.ajog.2013.03.017. View

10.

Peracoli J, Bannwart-Castro C, Romao M, Weel I, Ribeiro V, Borges V . High levels of heat shock protein 70 are associated with pro-inflammatory cytokines and may differentiate early- from late-onset preeclampsia. J Reprod Immunol. 2013; 100(2):129-34. DOI: 10.1016/j.jri.2013.08.003. View

11.

Chaiworapongsa T, Erez O, Kusanovic J, Vaisbuch E, Mazaki-Tovi S, Gotsch F . Amniotic fluid heat shock protein 70 concentration in histologic chorioamnionitis, term and preterm parturition. J Matern Fetal Neonatal Med. 2008; 21(7):449-61. PMC: 2517420. DOI: 10.1080/14767050802054550. View

12.

Osorio-Caballero M, Perdigon-Palacio C, Garcia-Lopez G, Flores-Herrera O, Olvera-Sanchez S, Morales-Mendez I . Escherichia coli-induced temporal and differential secretion of heat-shock protein 70 and interleukin-1β by human fetal membranes in a two-compartment culture system. Placenta. 2015; 36(3):262-9. DOI: 10.1016/j.placenta.2014.12.011. View

13.

Ferat-Osorio E, Sanchez-Anaya A, Gutierrez-Mendoza M, Bosco-Garate I, Wong-Baeza I, Pastelin-Palacios R . Heat shock protein 70 down-regulates the production of toll-like receptor-induced pro-inflammatory cytokines by a heat shock factor-1/constitutive heat shock element-binding factor-dependent mechanism. J Inflamm (Lond). 2014; 11:19. PMC: 4105516. DOI: 10.1186/1476-9255-11-19. View

14.

Haedersdal S, Salvig J, Aabye M, Thorball C, Ruhwald M, Ladelund S . Inflammatory markers in the second trimester prior to clinical onset of preeclampsia, intrauterine growth restriction, and spontaneous preterm birth. Inflammation. 2013; 36(4):907-13. DOI: 10.1007/s10753-013-9619-x. View

15.

Alvarez-Cabrera M, Barrientos-Galeana E, Barrera-Garcia A, Osorio-Caballero M, Acevedo J, Flores-Herrera O . Secretion of heat shock -60, -70 kD protein, IL-1β and TNFα levels in serum of a term normal pregnancy and patients with pre-eclampsia development. J Cell Mol Med. 2018; 22(11):5748-5752. PMC: 6201347. DOI: 10.1111/jcmm.13824. View

16.

. Hypertension in pregnancy. Report of the American College of Obstetricians and Gynecologists’ Task Force on Hypertension in Pregnancy. Obstet Gynecol. 2013; 122(5):1122-1131. DOI: 10.1097/01.AOG.0000437382.03963.88. View