Staphylococcal Biofilms

Overview

Journal Microbiol Spectr

Specialty Microbiology

Date 2018 Aug 18

PMID 30117414

Citations 154

Authors

Michael Otto

Affiliations

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Abstract

Staphylococci, with the leading species and , are the most frequent causes of infections on indwelling medical devices. The biofilm phenotype that those bacteria adopt during device-associated infection facilitates increased resistance to antibiotics and host immune defenses. This review presents and discusses the molecular mechanisms contributing to staphylococcal biofilm development and their in-vivo importance. Furthermore, it summarizes current strategies for the development of therapeutics against staphylococcal biofilm-associated infection.

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References

Cheung G, Joo H, Chatterjee S, Otto M . Phenol-soluble modulins--critical determinants of staphylococcal virulence. FEMS Microbiol Rev. 2013; 38(4):698-719. PMC: 4072763. DOI: 10.1111/1574-6976.12057. View

Raad I . Intravascular-catheter-related infections. Lancet. 1998; 351(9106):893-8. DOI: 10.1016/S0140-6736(97)10006-X. View

Conlon B, Rowe S, Lewis K . Persister cells in biofilm associated infections. Adv Exp Med Biol. 2014; 831:1-9. DOI: 10.1007/978-3-319-09782-4_1. View

Wittebole X, De Roock S, Opal S . A historical overview of bacteriophage therapy as an alternative to antibiotics for the treatment of bacterial pathogens. Virulence. 2013; 5(1):226-35. PMC: 3916379. DOI: 10.4161/viru.25991. View

Clarke S, Harris L, Geoff Richards R, Foster S . Analysis of Ebh, a 1.1-megadalton cell wall-associated fibronectin-binding protein of Staphylococcus aureus. Infect Immun. 2002; 70(12):6680-7. PMC: 133066. DOI: 10.1128/IAI.70.12.6680-6687.2002. View

Heinzelmann M, Herzig D, Swain B, Mercer-Jones M, Bergamini T, Polk Jr H . Phagocytosis and oxidative-burst response of planktonic Staphylococcus epidermidis RP62A and its non-slime-producing variant in human neutrophils. Clin Diagn Lab Immunol. 1998; 4(6):705-10. PMC: 170644. DOI: 10.1128/cdli.4.6.705-710.1997. View

Kokai-Kun J, Chanturiya T, Mond J . Lysostaphin eradicates established Staphylococcus aureus biofilms in jugular vein catheterized mice. J Antimicrob Chemother. 2009; 64(1):94-100. DOI: 10.1093/jac/dkp145. View

Ramasubbu N, Thomas L, Ragunath C, Kaplan J . Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans. J Mol Biol. 2005; 349(3):475-86. DOI: 10.1016/j.jmb.2005.03.082. View

Cerca N, Oliveira R, Azeredo J . Susceptibility of Staphylococcus epidermidis planktonic cells and biofilms to the lytic action of staphylococcus bacteriophage K. Lett Appl Microbiol. 2007; 45(3):313-7. DOI: 10.1111/j.1472-765X.2007.02190.x. View

10.

Schaffer A, Lee J . Staphylococcal vaccines and immunotherapies. Infect Dis Clin North Am. 2009; 23(1):153-71. DOI: 10.1016/j.idc.2008.10.005. View

11.

Hoffmann N, Rasmussen T, Jensen P, Stub C, Hentzer M, Molin S . Novel mouse model of chronic Pseudomonas aeruginosa lung infection mimicking cystic fibrosis. Infect Immun. 2005; 73(4):2504-14. PMC: 1087399. DOI: 10.1128/IAI.73.4.2504-2514.2005. View

12.

Formosa-Dague C, Speziale P, Foster T, Geoghegan J, Dufrene Y . Zinc-dependent mechanical properties of Staphylococcus aureus biofilm-forming surface protein SasG. Proc Natl Acad Sci U S A. 2015; 113(2):410-5. PMC: 4720321. DOI: 10.1073/pnas.1519265113. View

13.

Conrady D, Brescia C, Horii K, Weiss A, Hassett D, Herr A . A zinc-dependent adhesion module is responsible for intercellular adhesion in staphylococcal biofilms. Proc Natl Acad Sci U S A. 2008; 105(49):19456-61. PMC: 2592360. DOI: 10.1073/pnas.0807717105. View

14.

McCarthy H, Waters E, Bose J, Foster S, Bayles K, ONeill E . The major autolysin is redundant for Staphylococcus aureus USA300 LAC JE2 virulence in a murine device-related infection model. FEMS Microbiol Lett. 2016; 363(9). DOI: 10.1093/femsle/fnw087. View

15.

Loughran A, Atwood D, Anthony A, Harik N, Spencer H, Beenken K . Impact of individual extracellular proteases on Staphylococcus aureus biofilm formation in diverse clinical isolates and their isogenic sarA mutants. Microbiologyopen. 2014; 3(6):897-909. PMC: 4263513. DOI: 10.1002/mbo3.214. View

16.

Soe N, Jensen N, Jensen A, Koch J, Poulsen S, Pier G . Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats. In Vivo. 2017; 31(1):45-50. PMC: 5354146. DOI: 10.21873/invivo.11023. View

17.

Prabhakara R, Harro J, Leid J, Harris M, Shirtliff M . Murine immune response to a chronic Staphylococcus aureus biofilm infection. Infect Immun. 2011; 79(4):1789-96. PMC: 3067568. DOI: 10.1128/IAI.01386-10. View

18.

Heilmann C . Adhesion mechanisms of staphylococci. Adv Exp Med Biol. 2011; 715:105-23. DOI: 10.1007/978-94-007-0940-9_7. View

19.

Kies S, Otto M, Vuong C, Gotz F . Identification of the sigB operon in Staphylococcus epidermidis: construction and characterization of a sigB deletion mutant. Infect Immun. 2001; 69(12):7933-6. PMC: 98894. DOI: 10.1128/IAI.69.12.7933-7936.2001. View

20.

Bose J, Lehman M, Fey P, Bayles K . Contribution of the Staphylococcus aureus Atl AM and GL murein hydrolase activities in cell division, autolysis, and biofilm formation. PLoS One. 2012; 7(7):e42244. PMC: 3409170. DOI: 10.1371/journal.pone.0042244. View