and Filamentous Hemagglutinins Are Processed at Different Sites

Overview

Journal FEBS Open Bio

Specialty Biology

Date 2018 Aug 9

PMID 30087831

Citations 2

Authors

David Jurnecka

Petr Man

Peter Sebo

Ladislav Bumba

Affiliations

Soon will be listed here.

Abstract

Filamentous hemagglutinin (FHA) mediates adherence and plays an important role in lower respiratory tract infections by pathogenic . The mature FHA proteins of (Bp-FHA) and the (Bb-FHA) are generated by processing of the respective FhaB precursors by the autotransporter subtilisin-type protease SphB1. We have used bottom-up proteomics with differential O/O labeling and show that despite high-sequence conservation of the corresponding FhaB segments, the mature Bp-FHA (~ 230 kDa) and Bb-FHA (~ 243 kDa) proteins are processed at different sites of FhaB, after the Ala-2348 and Lys-2479 residues, respectively. Moreover, protease surface accessibility probing by on-column (on-line) digestion of the Bp-FHA and Bb-FHA proteins yielded different peptide patterns, revealing structural differences in the N-terminal and C-terminal domains of the Bp-FHA and Bb-FHA proteins. These data indicate specific structural variations between the highly homologous FHA proteins.

Citing Articles

Filamentous Hemagglutinin of Does Not Interact with the β Integrin CD11b/CD18.

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Precision Vaccine Development: Cues From Natural Immunity.

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