A Novel Laser Desorption/ionization Method Using Through Hole Porous Alumina Membranes

Overview

Journal Rapid Commun Mass Spectrom

Specialty Chemistry

Date 2018 Aug 5

PMID 30076645

Citations 5

Authors

Yasuhide Naito

Masahiro Kotani

Takayuki Ohmura

Affiliations

Soon will be listed here.

Abstract

Rationale: A novel matrix-free laser desorption/ionization method based on porous alumina membranes was developed. The porous alumina membranes have a two-dimensional (2D) ordered structure consisting of closely aligned straight through holes of sub-micron in diameter that are amenable to mass production by industrial fabrication processes.

Methods: Considering a balance between the ion generating efficiency and the mechanical strength of the membranes, the typical values for the hole diameter, open aperture ratio and membrane thickness were set to 200 nm, 50% and 5 μm, respectively. The membranes were coated with platinum on a single side that was exposed to the laser. Evaluation experiments were conducted on the feasibility of this membrane structure for an ionization method using a single peptide and mixed peptides and polyethylene glycol samples and a commercial matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometer in the positive ion mode.

Results: Results showed a softness of ionization and no sweet spot nature. The capillary action of the through holes with very high aspect ratio enables several loading protocols including sample impregnation from the surface opposite to the laser exposure side.

Conclusions: The feasibility study indicates that the through hole porous alumina membranes have several advantages in terms of usefulness over the conventional surface-assisted laser desorption ionization (SALDI) methods. The proposed novel ionization method is termed Desorption Ionization Using Through Hole Alumina Membrane (DIUTHAME).

Citing Articles

Matrix-Assisted Laser Desorption and Electrospray Ionization Tandem Mass Spectrometry of Microbial and Synthetic Biodegradable Polymers.

Rizzarelli P, Rapisarda M Polymers (Basel). 2023; 15(10).

PMID: 37242931 PMC: 10222159. DOI: 10.3390/polym15102356.

Mass Spectrometry Imaging.

Shimma S Mass Spectrom (Tokyo). 2022; 11(1):A0102.

PMID: 35291501 PMC: 8900255. DOI: 10.5702/massspectrometry.A0102.

Matrix-Free High-Resolution Atmospheric-Pressure SALDI Mass Spectrometry Imaging of Biological Samples Using Nanostructured DIUTHAME Membranes.

Muller M, Bhandari D, Spengler B Metabolites. 2021; 11(9).

PMID: 34564440 PMC: 8468348. DOI: 10.3390/metabo11090624.

Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane.

Enomoto H, Kotani M, Ohmura T Foods. 2020; 9(4).

PMID: 32244711 PMC: 7230831. DOI: 10.3390/foods9040408.

A novel laser desorption/ionization method using through hole porous alumina membranes.

Naito Y, Kotani M, Ohmura T Rapid Commun Mass Spectrom. 2018; 32(21):1851-1858.

PMID: 30076645 PMC: 6175246. DOI: 10.1002/rcm.8252.

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