MiR-184 Promotes Cell Proliferation in Tongue Squamous Cell Carcinoma by Targeting SOX7

Overview

Journal Oncol Lett

Specialty Oncology

Date 2018 Jul 17

PMID 30008922

Citations 7

Authors

Daiyun Chen

Junfu Li

Shunrong Li

Ping Han

Ning Li

Yi Wang

Shouqin Du

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate whether the miR-184 could regulate the proliferation of the tongue squamous cell carcinoma (TSCC) through sex-determining region Y-box 7 (SOX7) gene. miR-184 expression was upregulated in TSCC cell lines and tissues. MTT assay revealed that overexpression of miR-184 significantly promoted the proliferation of the TSCC cells . SOX7 was the direct target of miR-184 and luciferase reporter assay confirmed that miR-184 downregulated the expression of SOX7. MTT assay verified that knockdown of SOX7 remarkably promoted the proliferation of TSCC cells . miR-184 promoted the proliferation of TSCC by targeting SOX7. Taken together, our results provided a new potential therapeutic target for TSCC treatment.

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References

Zheng Z, Liu J, Yang Z, Wu L, Xie H, Jiang C . MicroRNA-452 promotes stem-like cells of hepatocellular carcinoma by inhibiting Sox7 involving Wnt/β-catenin signaling pathway. Oncotarget. 2016; 7(19):28000-12. PMC: 5053705. DOI: 10.18632/oncotarget.8584. View

Lefebvre V, Dumitriu B, Penzo-Mendez A, Han Y, Pallavi B . Control of cell fate and differentiation by Sry-related high-mobility-group box (Sox) transcription factors. Int J Biochem Cell Biol. 2007; 39(12):2195-214. PMC: 2080623. DOI: 10.1016/j.biocel.2007.05.019. View

Man C, Kan Fung T, Wan H, Cher C, Fan A, Ng N . Suppression of SOX7 by DNA methylation and its tumor suppressor function in acute myeloid leukemia. Blood. 2015; 125(25):3928-36. DOI: 10.1182/blood-2014-06-580993. View

Shiomi N, Mori M, Tsuji H, Imai T, Inoue H, Tateishi S . Human RAD18 is involved in S phase-specific single-strand break repair without PCNA monoubiquitination. Nucleic Acids Res. 2006; 35(2):e9. PMC: 1802632. DOI: 10.1093/nar/gkl979. View

Wu G, Li W, He W, Jiang N, Zhang G, Chen W . Mir-184 post-transcriptionally regulates SOX7 expression and promotes cell proliferation in human hepatocellular carcinoma. PLoS One. 2014; 9(2):e88796. PMC: 3928300. DOI: 10.1371/journal.pone.0088796. View

Rosebush M, Rao S, Samant S, Gu W, Handorf C, Pfeffer L . Oral cancer: enduring characteristics and emerging trends. J Mich Dent Assoc. 2012; 94(2):64-8. View

Ambros V . The functions of animal microRNAs. Nature. 2004; 431(7006):350-5. DOI: 10.1038/nature02871. View

Stovall D, Cao P, Sui G . SOX7: from a developmental regulator to an emerging tumor suppressor. Histol Histopathol. 2013; 29(4):439-45. PMC: 4107680. DOI: 10.14670/HH-29.10.439. View

Shen F, Cai W, Feng Z, Li J, Chen J, Cao J . MiR-492 contributes to cell proliferation and cell cycle of human breast cancer cells by suppressing SOX7 expression. Tumour Biol. 2014; 36(3):1913-21. DOI: 10.1007/s13277-014-2794-z. View

10.

Yu X, Li Z . MicroRNA expression and its implications for diagnosis and therapy of tongue squamous cell carcinoma. J Cell Mol Med. 2015; 20(1):10-6. PMC: 4717854. DOI: 10.1111/jcmm.12650. View

11.

Wong T, Liu X, Wong B, Ng R, Yuen A, Wei W . Mature miR-184 as Potential Oncogenic microRNA of Squamous Cell Carcinoma of Tongue. Clin Cancer Res. 2008; 14(9):2588-92. DOI: 10.1158/1078-0432.CCR-07-0666. View

12.

Tung M, Lin P, Cheng Y, Wu D, Yeh S, Chen C . Reduction of microRNA-184 by E6 oncoprotein confers cisplatin resistance in lung cancer via increasing Bcl-2. Oncotarget. 2016; 7(22):32362-74. PMC: 5078019. DOI: 10.18632/oncotarget.8708. View

13.

Zhen Y, Liu Z, Yang H, Yu X, Wu Q, Hua S . Tumor suppressor PDCD4 modulates miR-184-mediated direct suppression of C-MYC and BCL2 blocking cell growth and survival in nasopharyngeal carcinoma. Cell Death Dis. 2013; 4:e872. PMC: 3824685. DOI: 10.1038/cddis.2013.376. View

14.

Chen C, Mendez E, Houck J, Fan W, Lohavanichbutr P, Doody D . Gene expression profiling identifies genes predictive of oral squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev. 2008; 17(8):2152-62. PMC: 2575803. DOI: 10.1158/1055-9965.EPI-07-2893. View

15.

Wang W, Liu Z, Zhao L, Sun J, He Q, Yan W . Hexokinase 2 enhances the metastatic potential of tongue squamous cell carcinoma via the SOD2-H2O2 pathway. Oncotarget. 2016; 8(2):3344-3354. PMC: 5356886. DOI: 10.18632/oncotarget.13763. View

16.

Fang Z, Wu L, Wang L, Yang Y, Meng Y, Yang H . Increased expression of the long non-coding RNA UCA1 in tongue squamous cell carcinomas: a possible correlation with cancer metastasis. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013; 117(1):89-95. DOI: 10.1016/j.oooo.2013.09.007. View

17.

Harley V, Lovell-Badge R, Goodfellow P . Definition of a consensus DNA binding site for SRY. Nucleic Acids Res. 1994; 22(8):1500-1. PMC: 308012. DOI: 10.1093/nar/22.8.1500. View

18.

Calin G, Croce C . MicroRNA signatures in human cancers. Nat Rev Cancer. 2006; 6(11):857-66. DOI: 10.1038/nrc1997. View

19.

Emdad L, Janjic A, Alzubi M, Hu B, Santhekadur P, Menezes M . Suppression of miR-184 in malignant gliomas upregulates SND1 and promotes tumor aggressiveness. Neuro Oncol. 2014; 17(3):419-29. PMC: 4483100. DOI: 10.1093/neuonc/nou220. View

20.

Liu H, Yan Z, Li B, Yin S, Sun Q, Kou J . Reduced expression of SOX7 in ovarian cancer: a novel tumor suppressor through the Wnt/β-catenin signaling pathway. J Ovarian Res. 2014; 7:87. PMC: 4172779. DOI: 10.1186/s13048-014-0087-1. View