Genome-wide SWAp-Tag Yeast Libraries for Proteome Exploration

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2018 Jul 11

PMID 29988094

Citations 85

Authors

Uri Weill

Ido Yofe

Ehud Sass

Bram Stynen

Dan Davidi

Janani Natarajan

Reut Ben-Menachem

Zohar Avihou

Omer Goldman

Nofar Harpaz

Silvia Chuartzman

Kiril Kniazev

Barbara Knoblach

Janina Laborenz

Felix Boos

Jacqueline Kowarzyk

Shifra Ben-Dor

Einat Zalckvar

Johannes M Herrmann

Richard A Rachubinski

Ophry Pines

Doron Rapaport

Stephen W Michnick

Emmanuel D Levy

Maya Schuldiner

Affiliations

Soon will be listed here.

Abstract

Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

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