Antioxidant Defense Capacity of Ovarian Tissue After Vitrification in a Metal Closed System

Overview

Journal JBRA Assist Reprod

Specialty Reproductive Medicine

Date 2018 Jul 6

PMID 29975498

Citations 5

Authors

Eloisa T Massignam

Maite Ferreira

Eduardo Sanguinet

Agata Dupont

Fabio Klamt

Nilo Frantz

Adriana Bos-Mikich

Affiliations

Soon will be listed here.

Abstract

Objective: The present study analyzed the quality of bovine ovarian tissue after vitrification in a metal closed chamber, in terms of putative changes in tissue viability (lactate dehydrogenase -LDH- release), anti-oxidant defenses, and redox parameters caused by cryopreservation.

Methods: Small and large fragmented bovine ovarian tissue specimens were vitrified in a metal chamber. After rewarming, tissue samples were fixed or cultured for 48 hours. Glutathione (GSH), protein sulfhydryl content, Total Radical Trapping Antioxidant Potential (TRAP), and lactate dehydrogenase were analyzed immediately after rewarming and after tissue culture.

Results: No changes in antioxidant parameters or viability of rewarmed tissue samples were found immediately or 48h after vitrification. The method of vitrification in a metal closed chamber used in this study preserved the quality of bovine ovarian tissue. Furthermore, our data showed that the size of the tissue specimens did not affect post-vitrification biochemical viability parameters.

Conclusions: We believe that the vitrification methodology employed in the present study is safe and effective, and should be evaluated for use in humans.

Citing Articles

Static magnetic field assisted thawing improves cryopreservation of mouse whole ovaries.

Zhang L, Chi M, Cheng Y, Chen Z, Cao Y, Zhao G Bioeng Transl Med. 2024; 9(1):e10613.

PMID: 38193129 PMC: 10771557. DOI: 10.1002/btm2.10613.

Ovarian tissue cryopreservation and transplantation: a review on reactive oxygen species generation and antioxidant therapy.

Najafi A, Asadi E, Benson J Cell Tissue Res. 2023; 393(3):401-423.

PMID: 37328708 DOI: 10.1007/s00441-023-03794-2.

Comparison between two cryopreservation techniques of human ovarian cortex: morphological aspects and the heat shock response (HSR).

Galbinski S, Kowalewski L, Grigolo G, da Silva L, Jimenez M, Krause M Cell Stress Chaperones. 2022; 27(2):97-106.

PMID: 35043289 PMC: 8943117. DOI: 10.1007/s12192-022-01252-6.

Morphological study on the effects of sample size on ovarian tissue vitrification.

Dupont A, Sanguinet E, Ferreira M, Ramos L, Lothhammer N, Frantz N JBRA Assist Reprod. 2021; 26(3):374-378.

PMID: 34609111 PMC: 9355452. DOI: 10.5935/1518-0557.20210075.

Detailed Morphological Analysis of Cryoinjury in Human Ovarian Tissue Following Vitrification or Slow Freezing.

Ramos L, Galbinski S, Nacul A, Jimenez M, Frantz N, Bos-Mikich A Reprod Sci. 2021; 29(8):2374-2381.

PMID: 34398410 DOI: 10.1007/s43032-021-00716-x.

References

Zor T, SELINGER Z . Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Anal Biochem. 1996; 236(2):302-8. DOI: 10.1006/abio.1996.0171. View

Abedelahi A, Salehnia M, Allameh A, Davoodi D . Sodium selenite improves the in vitro follicular development by reducing the reactive oxygen species level and increasing the total antioxidant capacity and glutathione peroxide activity. Hum Reprod. 2010; 25(4):977-85. DOI: 10.1093/humrep/deq002. View

Ruder E, Hartman T, Blumberg J, Goldman M . Oxidative stress and antioxidants: exposure and impact on female fertility. Hum Reprod Update. 2008; 14(4):345-57. PMC: 2772106. DOI: 10.1093/humupd/dmn011. View

Isachenko V, Lapidus I, Isachenko E, Krivokharchenko A, Kreienberg R, Woriedh M . Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation. Reproduction. 2009; 138(2):319-27. DOI: 10.1530/REP-09-0039. View

Silva E, Greene A, Strauss K, Herrick J, Schoolcraft W, Krisher R . Antioxidant supplementation during in vitro culture improves mitochondrial function and development of embryos from aged female mice. Reprod Fertil Dev. 2015; 27(6):975-83. DOI: 10.1071/RD14474. View

Mazur P, Katkov I, Katkova N, Critser J . The enhancement of the ability of mouse sperm to survive freezing and thawing by the use of high concentrations of glycerol and the presence of an Escherichia coli membrane preparation (Oxyrase) to lower the oxygen concentration. Cryobiology. 2000; 40(3):187-209. DOI: 10.1006/cryo.2000.2238. View

Migishima F, Suzuki-Migishima R, Song S, Kuramochi T, Azuma S, Nishijima M . Successful cryopreservation of mouse ovaries by vitrification. Biol Reprod. 2003; 68(3):881-7. DOI: 10.1095/biolreprod.102.007948. View

Bielanski A, Nadin-Davis S, Sapp T . Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology. 2000; 40(2):110-6. DOI: 10.1006/cryo.1999.2227. View

Marques L, Bos-Mikich A, Godoy L, Silva L, Maschio D, Zhang T . Viability of zebrafish (Danio rerio) ovarian follicles after vitrification in a metal container. Cryobiology. 2015; 71(3):367-73. DOI: 10.1016/j.cryobiol.2015.09.004. View

10.

Xiao J, Liu Y, Li Z, Zhou Y, Lin H, Wu X . Effects of the insemination of hydrogen peroxide-treated epididymal mouse spermatozoa on γH2AX repair and embryo development. PLoS One. 2012; 7(6):e38742. PMC: 3383764. DOI: 10.1371/journal.pone.0038742. View

11.

Ferreira M, Bos-Mikich A, Frantz N, Rodrigues J, Brunetto A, Schwartsmann G . The effects of sample size on the outcome of ovarian tissue cryopreservation. Reprod Domest Anim. 2008; 45(1):99-102. DOI: 10.1111/j.1439-0531.2008.01261.x. View

12.

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

13.

Pomeroy K, Harris S, Conaghan J, Papadakis M, Centola G, Basuray R . Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk. Fertil Steril. 2009; 94(4):1181-1188. DOI: 10.1016/j.fertnstert.2009.04.031. View

14.

Lissi E, Pascual C, Del Castillo M . Evaluation of total antioxidant potential (TRAP) and total antioxidant reactivity from luminol-enhanced chemiluminescence measurements. Free Radic Biol Med. 1995; 18(2):153-8. DOI: 10.1016/0891-5849(94)00117-3. View

15.

Mazoochi T, Salehnia M, Pourbeiranvand S, Forouzandeh M, Mowla S, Hajizadeh E . Analysis of apoptosis and expression of genes related to apoptosis in cultures of follicles derived from vitrified and non-vitrified ovaries. Mol Hum Reprod. 2009; 15(3):155-64. DOI: 10.1093/molehr/gap002. View

16.

Vu H, Lee S, Acosta T, Yoshioka S, Abe H, Okuda K . Roles of prostaglandin F2alpha and hydrogen peroxide in the regulation of Copper/Zinc superoxide dismutase in bovine corpus luteum and luteal endothelial cells. Reprod Biol Endocrinol. 2012; 10:87. PMC: 3545964. DOI: 10.1186/1477-7827-10-87. View

17.

Preissler T, Bristot I, Costa B, Fernandes E, Rieger E, Bortoluzzi V . Phenylalanine induces oxidative stress and decreases the viability of rat astrocytes: possible relevance for the pathophysiology of neurodegeneration in phenylketonuria. Metab Brain Dis. 2015; 31(3):529-37. DOI: 10.1007/s11011-015-9763-0. View

18.

Keros V, Xella S, Hultenby K, Pettersson K, Sheikhi M, Volpe A . Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue. Hum Reprod. 2009; 24(7):1670-83. DOI: 10.1093/humrep/dep079. View

19.

Chen S, Chien C, Wu M, Chen T, Lai S, Lin C . Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice. Hum Reprod. 2006; 21(11):2794-800. DOI: 10.1093/humrep/del210. View

20.

Tokieda Y, Ishiwata I, Segino M, Ishikawa H, Sato K . Establishment of a novel method for cryopreservation and thawing of the mouse ovary. Hum Cell. 2003; 15(4):230-7. DOI: 10.1111/j.1749-0774.2002.tb00120.x. View