» Articles » PMID: 25739837

Antioxidant Supplementation During in Vitro Culture Improves Mitochondrial Function and Development of Embryos from Aged Female Mice

Overview
Date 2015 Mar 6
PMID 25739837
Citations 26
Authors
Affiliations
Soon will be listed here.
Abstract

Maternal aging results in reduced oocyte and blastocyst quality, thought to be due, in part, to mitochondrial dysfunction and accumulation of reactive oxygen species. To reduce oxidative stress, the antioxidants α-lipoic acid (ALA; 10µM), α-tocopherol (250µM), hypotaurine (1mM) and N-acetylcysteine (NAC; 1mM), and sirtuin (100ngmL(-1)) were added to embryo culture medium (AntiOX) and compared with a control (CON) without antioxidants to assess blastocyst development after in vitro maturation and fertilisation of oocytes from aged B6D2F1 female mice (13.5 months). Development to the blastocyst stage increased in the AntiOX compared with CON group (87.6% vs 72.7%, respectively; P<0.01), in addition to higher mitochondrial membrane potential and ATP levels in the AntiOX group. Expression of genes associated with oxidative stress (PI3K, FOXO3A and GLRX2) was upregulated in the CON compared with AntiOX group. In addition to AntiOX, a medium containing only NAC and ALA (rAntiOX) was used to culture embryos from young CF1 females (6-8 weeks). More blastocysts developed in the rAntiOX compared with CON group (64.1% vs 43.3%, respectively; P<0.01), although AntiOX (48.0% blastocysts) did not result in improved development in young mice. Antioxidants improved mitochondrial activity, gene expression and development in embryos of older female mice, whereas a reduced level of antioxidants during culture was beneficial to embryos from young mice.

Citing Articles

Mitochondrial dysfunction in pregnancy loss: a review.

Lu L, Huang X, Shi Y, Jiang Y, Han Y, Zhang Y Mol Cell Biochem. 2024; .

PMID: 39621222 DOI: 10.1007/s11010-024-05171-1.


Lipid Enriched Reduced Nutrient Culture Medium Improves Bovine Blastocyst Formation.

Pasquariello R, Zhang M, Herrick J, Ermisch A, Becker J, Schoolcraft W Reprod Fertil. 2023; .

PMID: 37971749 PMC: 10762584. DOI: 10.1530/RAF-23-0057.


Mating modifies the expression of crucial oxidative-reductive transcripts in the pig oviductal sperm reservoir: is the female ensuring sperm survival?.

Alvarez-Rodriguez M, Roca J, Martinez E, Rodriguez-Martinez H Front Endocrinol (Lausanne). 2023; 14:1042176.

PMID: 37351104 PMC: 10282951. DOI: 10.3389/fendo.2023.1042176.


Granulosa cell-derived extracellular vesicles mitigate the detrimental impact of thermal stress on bovine oocytes and embryos.

Menjivar N, Gad A, Gebremedhn S, Ghosh S, Tesfaye D Front Cell Dev Biol. 2023; 11:1142629.

PMID: 37091982 PMC: 10116072. DOI: 10.3389/fcell.2023.1142629.


Effects of N-acetylcysteine on Growth, Viability, and Ultrastructure of In Vitro Cultured Bovine Secondary Follicles.

Nascimento D, Azevedo V, Barroso P, Barrozo L, Silva B, Silva A Animals (Basel). 2022; 12(22).

PMID: 36428416 PMC: 9687016. DOI: 10.3390/ani12223190.