1α,25-dihydroxyvitamin D-eluting Nanofibrous Dressings Induce Endogenous Antimicrobial Peptide Expression

Overview

Journal Nanomedicine (Lond)

Specialty Biotechnology

Date 2018 Jul 5

PMID 29972648

Citations 12

Authors

Jiang Jiang

Yang Zhang

Arup K Indra

Gitali Ganguli-Indra

Mai N Le

Hongjun Wang

Ronald R Hollins

Debra A Reilly

Mark A Carlson

Richard L Gallo

Adrian F Gombart

Jingwei Xie

Affiliations

Soon will be listed here.

Abstract

Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D (1,25(OH)D) and augmenting human CAMP induction.

Materials & Methods: Nanofibrous wound dressings containing 1,25(OH)D were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo.

Results: 1,25(OH)D was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)D showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)D-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants.

Conclusion: 1,25(OH)D containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

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