MiR-133b Targets Sox9 to Control Pathogenesis and Metastasis of Breast Cancer

Overview

Journal Cell Death Dis

Specialties Cell Biology
General Medicine

Date 2018 Jul 5

PMID 29970901

Citations 33

Authors

Qiu-Yu Wang

Ci-Xiang Zhou

Meng-Na Zhan

Jun Tang

Chen-Long Wang

Cheng-Ning Ma

Ming He

Guo-Qiang Chen

Jian-Rong He

Qian Zhao

Affiliations

Soon will be listed here.

Abstract

The miR-133b, a commonly recognized muscle-specific miRNA, was reported to be deregulated in many kinds of cancers. However, its potential roles in tumorigenesis remain greatly elusive. Herein, we demonstrate that miR-133b is significantly suppressed in human breast cancer specimens, which is reversely correlated to histological grade of the cancer. Ectopic expression of miR-133b suppresses clonogenic ability and metastasis-relevant traits in vitro, as well as carcinogenesis and pulmonary metastasis in vivo. Further studies have identified Sox9, c-MET, and WAVE2 as direct targets of miR-133b, in which Sox9 contributes to all miR-133b-endowed effects including cell proliferation, colony formation, as well as cell migration and invasion in vitro. Moreover, re-expression of Sox9 reverses miR-133b-mediated metastasis suppression in vivo. Taken together, these findings highlight an important role for miR-133b in the regulation of tumorigenesis and metastatic potential of breast cancer and suggest a potential application of miR-133b in cancer treatment.

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References

Shimono Y, Zabala M, Cho R, Lobo N, Dalerba P, Qian D . Downregulation of miRNA-200c links breast cancer stem cells with normal stem cells. Cell. 2009; 138(3):592-603. PMC: 2731699. DOI: 10.1016/j.cell.2009.07.011. View

Trajkovski M, Ahmed K, Esau C, Stoffel M . MyomiR-133 regulates brown fat differentiation through Prdm16. Nat Cell Biol. 2012; 14(12):1330-5. DOI: 10.1038/ncb2612. View

Qin W, Dong P, Ma C, Mitchelson K, Deng T, Zhang L . MicroRNA-133b is a key promoter of cervical carcinoma development through the activation of the ERK and AKT1 pathways. Oncogene. 2011; 31(36):4067-75. DOI: 10.1038/onc.2011.561. View

Chaffer C, Weinberg R . A perspective on cancer cell metastasis. Science. 2011; 331(6024):1559-64. DOI: 10.1126/science.1203543. View

Kim J, Inoue K, Ishii J, Vanti W, Voronov S, Murchison E . A MicroRNA feedback circuit in midbrain dopamine neurons. Science. 2007; 317(5842):1220-4. PMC: 2782470. DOI: 10.1126/science.1140481. View

Sanchez-Simon F, Zhang X, Loh H, Law P, Rodriguez R . Morphine regulates dopaminergic neuron differentiation via miR-133b. Mol Pharmacol. 2010; 78(5):935-42. PMC: 2981367. DOI: 10.1124/mol.110.066837. View

Valastyan S, Weinberg R . Tumor metastasis: molecular insights and evolving paradigms. Cell. 2011; 147(2):275-92. PMC: 3261217. DOI: 10.1016/j.cell.2011.09.024. View

Perou C, Sorlie T, Eisen M, van de Rijn M, Jeffrey S, Rees C . Molecular portraits of human breast tumours. Nature. 2000; 406(6797):747-52. DOI: 10.1038/35021093. View

Townley-Tilson W, Callis T, Wang D . MicroRNAs 1, 133, and 206: critical factors of skeletal and cardiac muscle development, function, and disease. Int J Biochem Cell Biol. 2010; 42(8):1252-5. PMC: 2904322. DOI: 10.1016/j.biocel.2009.03.002. View

10.

Stolt C, Lommes P, Sock E, Chaboissier M, Schedl A, Wegner M . The Sox9 transcription factor determines glial fate choice in the developing spinal cord. Genes Dev. 2003; 17(13):1677-89. PMC: 196138. DOI: 10.1101/gad.259003. View

11.

Grimont A, Pinho A, Cowley M, Augereau C, Mawson A, Giry-Laterriere M . SOX9 regulates ERBB signalling in pancreatic cancer development. Gut. 2014; 64(11):1790-9. DOI: 10.1136/gutjnl-2014-307075. View

12.

Chaboissier M, Kobayashi A, Vidal V, Lutzkendorf S, van de Kant H, Wegner M . Functional analysis of Sox8 and Sox9 during sex determination in the mouse. Development. 2004; 131(9):1891-901. DOI: 10.1242/dev.01087. View

13.

Hermann P, Huber S, Herrler T, Aicher A, Ellwart J, Guba M . Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer. Cell Stem Cell. 2008; 1(3):313-23. DOI: 10.1016/j.stem.2007.06.002. View

14.

Kordes U, Hagel C . Expression of SOX9 and SOX10 in central neuroepithelial tumor. J Neurooncol. 2006; 80(2):151-5. DOI: 10.1007/s11060-006-9180-7. View

15.

Kano M, Seki N, Kikkawa N, Fujimura L, Hoshino I, Akutsu Y . miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma. Int J Cancer. 2011; 127(12):2804-14. DOI: 10.1002/ijc.25284. View

16.

Ventura A, Jacks T . MicroRNAs and cancer: short RNAs go a long way. Cell. 2009; 136(4):586-91. PMC: 3910108. DOI: 10.1016/j.cell.2009.02.005. View

17.

Gupta G, Massague J . Cancer metastasis: building a framework. Cell. 2006; 127(4):679-95. DOI: 10.1016/j.cell.2006.11.001. View

18.

Pang R, Lun Law W, Chu A, Poon J, Lam C, Chow A . A subpopulation of CD26+ cancer stem cells with metastatic capacity in human colorectal cancer. Cell Stem Cell. 2010; 6(6):603-15. DOI: 10.1016/j.stem.2010.04.001. View

19.

Akiyama H, Chaboissier M, Martin J, Schedl A, de Crombrugghe B . The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev. 2002; 16(21):2813-28. PMC: 187468. DOI: 10.1101/gad.1017802. View

20.

Shin S, Freedman V, Risser R, Pollack R . Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro. Proc Natl Acad Sci U S A. 1975; 72(11):4435-9. PMC: 388736. DOI: 10.1073/pnas.72.11.4435. View