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Insertion of DNA Sequences into the Human Chromosomal Beta-globin Locus by Homologous Recombination

Overview
Journal Nature
Specialty Science
Date 1985 Sep 19
PMID 2995814
Citations 310
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Abstract

A 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.

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