The HexB Mismatch Repair Gene of Streptococcus Pneumoniae: Characterisation, Cloning and Identification of the Product

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1985 Jan 1

PMID 2995767

Citations 18

Authors

H Prats

B Martin

J P Claverys

Affiliations

Soon will be listed here.

Abstract

A second gene involved in mismatch repair in Streptococcus pneumoniae, the hexB gene, has been characterised. The gene was cloned into a multicopy plasmid vector. The cloned hexB gene is expressed as judged by its ability to complement a chromosomal hexB- allele. Its direction of transcription and its functional limits were defined. Comparison of the proteins encoded by recombinant plasmids and by restriction fragments allowed us to identify an Mr 83,000 protein as the probable product of the hexB gene. We offer evidence that this gene together with the hexA gene is essential for repair of transition as well as frameshift mismatches.

Citing Articles

Phylloquinone improves endothelial function, inhibits cellular senescence, and vascular inflammation.

Kieronska-Rudek A, Kij A, Bar A, Kurpinska A, Mohaissen T, Grosicki M Geroscience. 2024; 46(5):4909-4935.

PMID: 38980631 PMC: 11336140. DOI: 10.1007/s11357-024-01225-w.

A high-resolution view of genome-wide pneumococcal transformation.

Croucher N, Harris S, Barquist L, Parkhill J, Bentley S PLoS Pathog. 2012; 8(6):e1002745.

PMID: 22719250 PMC: 3375284. DOI: 10.1371/journal.ppat.1002745.

Search for genes essential for pneumococcal transformation: the RADA DNA repair protein plays a role in genomic recombination of donor DNA.

Burghout P, Bootsma H, Kloosterman T, Bijlsma J, de Jongh C, Kuipers O J Bacteriol. 2007; 189(18):6540-50.

PMID: 17631629 PMC: 2045161. DOI: 10.1128/JB.00573-07.

Polarity of recombination in transformation of Streptococcus pneumoniae.

Pasta F, SICARD M Proc Natl Acad Sci U S A. 1999; 96(6):2943-8.

PMID: 10077616 PMC: 15874. DOI: 10.1073/pnas.96.6.2943.

The yeast gene MSH3 defines a new class of eukaryotic MutS homologues.

New L, Liu K, Crouse G Mol Gen Genet. 1993; 239(1-2):97-108.

PMID: 8510668 DOI: 10.1007/BF00281607.

References

Claverys J, Prats H, Vasseghi H, Gherardi M . Identification of Streptococcus pneumoniae mismatch repair genes by an additive transformation approach. Mol Gen Genet. 1984; 196(1):91-6. DOI: 10.1007/BF00334098. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Tiraby J, Fox M . Marker discrimination in transformation and mutation of pneumococcus. Proc Natl Acad Sci U S A. 1973; 70(12):3541-5. PMC: 427276. DOI: 10.1073/pnas.70.12.3541. View

Tiraby G, SICARD M . Integration efficiencies of spontaneous mutant alleles of amiA locus in pneumococcal transformation. J Bacteriol. 1973; 116(3):1130-5. PMC: 246466. DOI: 10.1128/jb.116.3.1130-1135.1973. View

Claverys J, Lefevre J, Sicard A . Transformation of Streptococcus pneumoniae with S. pneumoniae-lambda phage hybrid DNA: induction of deletions. Proc Natl Acad Sci U S A. 1980; 77(6):3534-8. PMC: 349651. DOI: 10.1073/pnas.77.6.3534. View

Martin B, Prats H, Claverys J . Cloning of the hexA mismatch-repair gene of Streptococcus pneumoniae and identification of the product. Gene. 1985; 34(2-3):293-303. DOI: 10.1016/0378-1119(85)90138-6. View

Claverys J, Louarn J, Sicard A . Cloning of Streptococcus pneumoniae DNA: its use in pneumococcal transformation and in studies of mismatch repair. Gene. 1981; 13(1):65-73. DOI: 10.1016/0378-1119(81)90044-5. View

Lacks S . Integration efficiency and genetic recombination in pneumococcal transformation. Genetics. 1966; 53(1):207-35. PMC: 1211003. DOI: 10.1093/genetics/53.1.207. View

10.

Thuring R, Sanders J, Borst P . A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem. 1975; 66(1):213-20. DOI: 10.1016/0003-2697(75)90739-3. View

11.

Vasseghi H, Claverys J . Amplification of a chimeric plasmid carrying an erythromycin-resistance determinant introduced into the genome of Streptococcus pneumoniae. Gene. 1983; 21(3):285-92. DOI: 10.1016/0378-1119(83)90012-4. View

12.

Wieslander L . A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal Biochem. 1979; 98(2):305-9. DOI: 10.1016/0003-2697(79)90145-3. View

13.

Claverys J, Mejean V, Gasc A, Sicard A . Mismatch repair in Streptococcus pneumoniae: relationship between base mismatches and transformation efficiencies. Proc Natl Acad Sci U S A. 1983; 80(19):5956-60. PMC: 390196. DOI: 10.1073/pnas.80.19.5956. View

14.

Burdett V . Identification of tetracycline-resistant R-plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 1980; 18(5):753-60. PMC: 284087. DOI: 10.1128/AAC.18.5.753. View

15.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

16.

Claverys J, Mejean V, Gasc A, Galibert F, Sicard A . Base specificity of mismatch repair in Streptococcus pneumoniae. Nucleic Acids Res. 1981; 9(10):2267-80. PMC: 326844. DOI: 10.1093/nar/9.10.2267. View

17.

Bolivar F . Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978; 4(2):121-36. DOI: 10.1016/0378-1119(78)90025-2. View

18.

Ephrussi-Taylor H, Sicard A, Kamen R . Genetic Recombination in DNA-Induced Transformation of Pneumococcus. I. the Problem of Relative Efficiency of Transforming Factors. Genetics. 1965; 51(3):455-75. PMC: 1210798. DOI: 10.1093/genetics/51.3.455. View

19.

Zubay G . In vitro synthesis of protein in microbial systems. Annu Rev Genet. 1973; 7:267-87. DOI: 10.1146/annurev.ge.07.120173.001411. View

20.

Lefevre J, Claverys J, Sicard A . Donor deoxyribonucleic acid length and marker effect in pneumococcal transformation. J Bacteriol. 1979; 138(1):80-6. PMC: 218240. DOI: 10.1128/jb.138.1.80-86.1979. View