The Yeast Gene MSH3 Defines a New Class of Eukaryotic MutS Homologues

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1993 May 1

PMID 8510668

Citations 48

Authors

L New

K Liu

G F Crouse

Affiliations

Soon will be listed here.

Abstract

We have identified a gene in Saccharomyces cerevisiae, MSH3, whose predicted protein product shares extensive sequence similarity with bacterial proteins involved in DNA mismatch repair as well as with the predicted protein product of the Rep-3 gene of mouse. MSH3 was obtained by performing a polymerase chain reaction on yeast genomic DNA using degenerate oligonucleotide primers designed to anneal with the most conserved regions of a gene that would be homologous to Rep-3 and Salmonella typhimurium mutS. MSH3 seems to play some role in DNA mismatch repair, inasmuch as its inactivation results in an increase in reversion rates of two different mutations and also causes an increase in postmeiotic segregation. However, the effect of MSH3 disruption on reversion rates and postmeiotic segregation appears to be much less than that of previously characterized yeast DNA mismatch repair genes. Alignment of the MSH3 sequence with all of the known MutS homologues suggests that its primary function may be different from the role of MutS in repair of replication errors. MSH3 appears to be more closely related to the mouse Rep-3 gene and other similar eukaryotic mutS homologues than to the yeast gene MSH2 and other mutS homologues that are involved in replication repair. We suggest that the primary function of MSH3 may be more closely related to one of the other known functions of mutS, such as its role in preventing recombination between non-identical sequences.

Citing Articles

Evolutionary Significance of Fungal Hypermutators: Lessons Learned from Clinical Strains and Implications for Fungal Plant Pathogens.

Gambhir N, Harris S, Everhart S mSphere. 2022; 7(3):e0008722.

PMID: 35638358 PMC: 9241500. DOI: 10.1128/msphere.00087-22.

A role for synaptonemal complex in meiotic mismatch repair.

Voelkel-Meiman K, Oke A, Feil A, Shames A, Fung J, MacQueen A Genetics. 2022; 220(2).

PMID: 35100397 PMC: 9097268. DOI: 10.1093/genetics/iyab230.

PCNA antagonizes cohesin-dependent roles in genomic stability.

Zuilkoski C, Skibbens R PLoS One. 2020; 15(10):e0235103.

PMID: 33075068 PMC: 7571713. DOI: 10.1371/journal.pone.0235103.

Interactome Analysis and Docking Sites of MutS Homologs Reveal New Physiological Roles in .

AbdelGawwad M, Maric A, Al-Ghamdi A, Hatamleh A Molecules. 2019; 24(13).

PMID: 31288414 PMC: 6651420. DOI: 10.3390/molecules24132493.

A personal historical view of DNA mismatch repair with an emphasis on eukaryotic DNA mismatch repair.

Kolodner R DNA Repair (Amst). 2015; 38:3-13.

PMID: 26698650 PMC: 4740188. DOI: 10.1016/j.dnarep.2015.11.009.

References

Claverys J, Prats H, Vasseghi H, Gherardi M . Identification of Streptococcus pneumoniae mismatch repair genes by an additive transformation approach. Mol Gen Genet. 1984; 196(1):91-6. DOI: 10.1007/BF00334098. View

Frischauf A, Lehrach H, Poustka A, Murray N . Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983; 170(4):827-42. DOI: 10.1016/s0022-2836(83)80190-9. View

Lahue R, Au K, Modrich P . DNA mismatch correction in a defined system. Science. 1989; 245(4914):160-4. DOI: 10.1126/science.2665076. View

Williamson M, Game J, Fogel S . Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics. 1985; 110(4):609-46. PMC: 1202584. DOI: 10.1093/genetics/110.4.609. View

Jiricny J, Hughes M, Corman N, Rudkin B . A human 200-kDa protein binds selectively to DNA fragments containing G.T mismatches. Proc Natl Acad Sci U S A. 1988; 85(23):8860-4. PMC: 282606. DOI: 10.1073/pnas.85.23.8860. View

Sherman F . Getting started with yeast. Methods Enzymol. 1991; 194:3-21. DOI: 10.1016/0076-6879(91)94004-v. View

Gorbalenya A, Koonin E . Superfamily of UvrA-related NTP-binding proteins. Implications for rational classification of recombination/repair systems. J Mol Biol. 1990; 213(4):583-91. DOI: 10.1016/S0022-2836(05)80243-8. View

Rayssiguier C, Thaler D, Radman M . The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature. 1989; 342(6248):396-401. DOI: 10.1038/342396a0. View

Reenan R, Kolodner R . Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins. Genetics. 1992; 132(4):963-73. PMC: 1205252. DOI: 10.1093/genetics/132.4.963. View

10.

LEA D, COULSON C . The distribution of the numbers of mutants in bacterial populations. J Genet. 2014; 49(3):264-85. DOI: 10.1007/BF02986080. View

11.

Brooks P, Dohet C, Almouzni G, Mechali M, Radman M . Mismatch repair involving localized DNA synthesis in extracts of Xenopus eggs. Proc Natl Acad Sci U S A. 1989; 86(12):4425-9. PMC: 287282. DOI: 10.1073/pnas.86.12.4425. View

12.

Hoffman C, Winston F . A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene. 1987; 57(2-3):267-72. DOI: 10.1016/0378-1119(87)90131-4. View

13.

Grilley M, Holmes J, Yashar B, Modrich P . Mechanisms of DNA-mismatch correction. Mutat Res. 1990; 236(2-3):253-67. DOI: 10.1016/0921-8777(90)90009-t. View

14.

Rothstein R . Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 1991; 194:281-301. DOI: 10.1016/0076-6879(91)94022-5. View

15.

Steele D . An examination of adaptive reversion in Saccharomyces cerevisiae. Genetics. 1992; 132(1):9-21. PMC: 1205133. DOI: 10.1093/genetics/132.1.9. View

16.

Walker J, Saraste M, Runswick M, Gay N . Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982; 1(8):945-51. PMC: 553140. DOI: 10.1002/j.1460-2075.1982.tb01276.x. View

17.

Haber L, Pang P, Sobell D, Mankovich J, Walker G . Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae. J Bacteriol. 1988; 170(1):197-202. PMC: 210626. DOI: 10.1128/jb.170.1.197-202.1988. View

18.

Mankovich J, McIntyre C, Walker G . Nucleotide sequence of the Salmonella typhimurium mutL gene required for mismatch repair: homology of MutL to HexB of Streptococcus pneumoniae and to PMS1 of the yeast Saccharomyces cerevisiae. J Bacteriol. 1989; 171(10):5325-31. PMC: 210369. DOI: 10.1128/jb.171.10.5325-5331.1989. View

19.

Smith A, Kalderon D, Roberts B, Colledge W, Edge M, Gillett P . The nuclear location signal. Proc R Soc Lond B Biol Sci. 1985; 226(1242):43-58. DOI: 10.1098/rspb.1985.0078. View

20.

Martin B, Prats H, Claverys J . Cloning of the hexA mismatch-repair gene of Streptococcus pneumoniae and identification of the product. Gene. 1985; 34(2-3):293-303. DOI: 10.1016/0378-1119(85)90138-6. View