A Method for Single-neuron Chronic Recording from the Retina in Awake Mice

Overview

Journal Science

Specialty Science

Date 2018 Jun 30

PMID 29954976

Citations 63

Authors

Guosong Hong

Tian-Ming Fu

Mu Qiao

Robert D Viveros

Xiao Yang

Tao Zhou

Jung Min Lee

Hong-Gyu Park

Joshua R Sanes

Charles M Lieber

Affiliations

Soon will be listed here.

Abstract

The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.

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