Cloning of CDNA Encoding a 100 KDa Nucleolar Protein (nucleoline) of Chinese Hamster Ovary Cells

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1985 Aug 26

PMID 2994013

Citations 5

Authors

B Lapeyre

M Caizergues-Ferrer

G Bouche

F Amalric

Affiliations

Soon will be listed here.

Abstract

Nucleoline (100 kDa) is the major nucleolar protein in exponentially growing cells that behaves like a nucleolar organizer protein and plays a key role in rDNA transcription and prerRNA processing. We reported the isolation of 5 cDNA clones by probing a cDNA library, constructed in the expression vector lambda gt11, with a polyclonal serum raised against nucleoline. A new immunoassay, using hybrid proteins (beta gal-cDNA encoded protein) was developed to establish that the isolated cDNAs encoded parts of nucleoline. A further confirmation resulted from the sequence comparison between the cDNA encoded peptide and a 42 aa peptide isolated from rat nucleoline (1). The 5 cDNAs overlapped extensively and covered more than 90% of a full length cDNA. By probing a Northern blot with the 100 kDa cDNA, a 2650 nucleotide polyA+ RNA was detected that contained just enough information to code for nucleoline.

Citing Articles

Lamprey lymphocyte-like cells express homologs of genes involved in immunologically relevant activities of mammalian lymphocytes.

Uinuk-Ool T, Mayer W, Sato A, Dongak R, Cooper M, Klein J Proc Natl Acad Sci U S A. 2002; 99(22):14356-61.

PMID: 12391333 PMC: 137888. DOI: 10.1073/pnas.212527699.

DNA binding properties of a 110 kDa nucleolar protein.

Sapp M, Knippers R, Richter A Nucleic Acids Res. 1986; 14(17):6803-20.

PMID: 3763392 PMC: 311700. DOI: 10.1093/nar/14.17.6803.

Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence.

Lapeyre B, Bourbon H, Amalric F Proc Natl Acad Sci U S A. 1987; 84(6):1472-6.

PMID: 3470736 PMC: 304456. DOI: 10.1073/pnas.84.6.1472.

A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.

Schimmang T, Tollervey D, Kern H, Frank R, Hurt E EMBO J. 1989; 8(13):4015-24.

PMID: 2686980 PMC: 401576. DOI: 10.1002/j.1460-2075.1989.tb08584.x.

Distribution of nucleolar proteins B23 and nucleolin during mouse spermatogenesis.

Biggiogera M, Kaufmann S, Shaper J, Gas N, Amalric F, Fakan S Chromosoma. 1991; 100(3):162-72.

PMID: 1710179 DOI: 10.1007/BF00337245.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Teem J, Abovich N, Kaufer N, Schwindinger W, Warner J, Levy A . A comparison of yeast ribosomal protein gene DNA sequences. Nucleic Acids Res. 1984; 12(22):8295-312. PMC: 320358. DOI: 10.1093/nar/12.22.8295. View

Caboche M, Bachellerie J . RNA methylation and control of eukaryotic RNA biosynthesis. Effects of cycloleucine, a specific inhibitor of methylation, on ribosomal RNA maturation. Eur J Biochem. 1977; 74(1):19-29. DOI: 10.1111/j.1432-1033.1977.tb11362.x. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Jacq B, Jourdan R, Jordan B . Structure and processing of precursor 5 S RNA in Drosophila melanogaster. J Mol Biol. 1977; 117(3):785-95. DOI: 10.1016/0022-2836(77)90069-9. View

Maniatis T, Hardison R, Lacy E, Lauer J, OConnell C, Quon D . The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978; 15(2):687-701. DOI: 10.1016/0092-8674(78)90036-3. View

Mamrack M, Olson M, Busch H . Amino acid sequence and sites of phosphorylation in a highly acidic region of nucleolar nonhistone protein C23. Biochemistry. 1979; 18(15):3381-6. DOI: 10.1021/bi00582a026. View

Long E, Dawid I . Repeated genes in eukaryotes. Annu Rev Biochem. 1980; 49:727-64. DOI: 10.1146/annurev.bi.49.070180.003455. View

Tsutsui K, Oda T . Isolation and characterization of a high-molecular-weight acid-soluble nuclear protein from mouse ascites-sarcoma cells. Eur J Biochem. 1980; 108(2):497-505. DOI: 10.1111/j.1432-1033.1980.tb04747.x. View

10.

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

11.

Bozzoni I, Beccari E, Luo Z, Amaldi F . Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization. Nucleic Acids Res. 1981; 9(5):1069-86. PMC: 326737. DOI: 10.1093/nar/9.5.1069. View

12.

DEustachio P, Meyuhas O, Ruddle F, PERRY R . Chromosomal distribution of ribosomal protein genes in the mouse. Cell. 1981; 24(2):307-12. DOI: 10.1016/0092-8674(81)90320-2. View

13.

Chooi W, Leiby K . An electron microscopic method for localization of ribosomal proteins during transcription of ribosomal DNA: a method for studying protein assembly. Proc Natl Acad Sci U S A. 1981; 78(8):4823-7. PMC: 320262. DOI: 10.1073/pnas.78.8.4823. View

14.

Woolford Jr J, Rosbash M . Ribosomal protein genes rp 39(10 - 78), rp 39(11 - 40), rp 51, and rp 52 are not contiguous to other ribosomal protein genes in the Saccharomyces cerevisiae genome. Nucleic Acids Res. 1981; 9(19):5021-36. PMC: 327496. DOI: 10.1093/nar/9.19.5021. View

15.

Moss T . Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter. Cell. 1982; 30(3):835-42. DOI: 10.1016/0092-8674(82)90288-4. View

16.

Rao S, Mamrack M, Olson M . Localization of phosphorylated highly acidic regions in the NH2-terminal half of nucleolar protein C23. J Biol Chem. 1982; 257(24):15035-41. View

17.

Grummt I . Nucleotide sequence requirements for specific initiation of transcription by RNA polymerase I. Proc Natl Acad Sci U S A. 1982; 79(22):6908-11. PMC: 347243. DOI: 10.1073/pnas.79.22.6908. View

18.

Bugler B, Caizergues-Ferrer M, Bouche G, Bourbon H, Amalric F . Detection and localization of a class of proteins immunologically related to a 100-kDa nucleolar protein. Eur J Biochem. 1982; 128(2-3):475-80. DOI: 10.1111/j.1432-1033.1982.tb06989.x. View

19.

Sakonju S, Brown D . Contact points between a positive transcription factor and the Xenopus 5S RNA gene. Cell. 1982; 31(2 Pt 1):395-405. DOI: 10.1016/0092-8674(82)90133-7. View

20.

Young R, Davis R . Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983; 80(5):1194-8. PMC: 393560. DOI: 10.1073/pnas.80.5.1194. View