Xenopus Laevis Ribosomal Protein Genes: Isolation of Recombinant CDNA Clones and Study of the Genomic Organization

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1981 Mar 11

PMID 6112733

Citations 35

Authors

I Bozzoni

E Beccari

Z X Luo

F Amaldi

Affiliations

Soon will be listed here.

Abstract

Poly-A+ mRNA from Xenopus laevis oocytes, partially enriched for r-protein coding capacity has been used as starting material for preparing a cDNA bank in plasmid pBR322. The clones containing sequences specific for r-proteins have been selected by translation of the complementary mRNAs. Clones for six different r-proteins have been identified and utilized as probes for studying their genomic organization. Two gene copies per haploid genome were found for r-proteins L1, L14, S19, and four-five for protein S1, S8 and L32. Moreover a population polymorphism has been observed for the genomic regions containing sequences for r-protein S1, S8 and L14.

Citing Articles

The primary sequence of the Schizosaccharomyces pombe protein homologous to S.cerevisiae ribosomal protein L2.

Presutti C, Villa T, Bozzoni I Nucleic Acids Res. 1993; 21(16):3900.

PMID: 8367312 PMC: 309927. DOI: 10.1093/nar/21.16.3900.

A novel small nucleolar RNA (U16) is encoded inside a ribosomal protein intron and originates by processing of the pre-mRNA.

Fragapane P, Prislei S, Michienzi A, Caffarelli E, Bozzoni I EMBO J. 1993; 12(7):2921-8.

PMID: 8335006 PMC: 413545. DOI: 10.1002/j.1460-2075.1993.tb05954.x.

In vitro study of processing of the intron-encoded U16 small nucleolar RNA in Xenopus laevis.

Caffarelli E, Arese M, Santoro B, Fragapane P, Bozzoni I Mol Cell Biol. 1994; 14(5):2966-74.

PMID: 7513048 PMC: 358664. DOI: 10.1128/mcb.14.5.2966-2974.1994.

Two different snoRNAs are encoded in introns of amphibian and human L1 ribosomal protein genes.

Prislei S, Michienzi A, Presutti C, Fragapane P, Bozzoni I Nucleic Acids Res. 1993; 21(25):5824-30.

PMID: 7507233 PMC: 310460. DOI: 10.1093/nar/21.25.5824.

Xenopus fibrinogen: characterization of the mRNAs for the three subunits.

Holland L, Wangh L Mol Cell Biol. 1984; 4(11):2543-8.

PMID: 6513931 PMC: 369088. DOI: 10.1128/mcb.4.11.2543-2548.1984.

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