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In Vitro Studies on Lymphocyte Differentiation. I. Long Term in Vitro Culture of Cells Giving Rise to Functional Lymphocytes in Irradiated Mice

Overview
Journal J Exp Med
Specialties Allergy & Immunology
General Medicine
Date 1978 Sep 1
PMID 29938
Citations 14
Authors
J W Schrader
S Schrader
Affiliations
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Abstract

In vitro cultures of mouse bone marrow cells, maintained for periods up to 7 wk, were shown to contain cells able to repopulate irradiated hosts with T and B lymphocytes. The lymphocytes were fully functional and there did not appear to be any gross restriction of their receptor repertoire. The cultured cells reconstituted irradiated semiallogenic hosts without evidence of graft-versus-host disease, suggesting that culture of donor marrow might be a useful preliminary to transplantation when tissue matching is incomplete.

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Long-term culture of B lymphocytes and their precursors from murine bone marrow.

Whitlock C, Witte O Proc Natl Acad Sci U S A. 1982; 79(11):3608-12.

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An investigation into the B lymphopoietic capacity of long-term bone marrow cultures.

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