The Effect of on the Expression Levels of Toll-like Receptor 2 and Interleukin-8 in HaCaT Cells Under High- and Low-glucose Conditions

Overview

Journal Indian J Dermatol

Specialty Dermatology

Date 2018 Jun 26

PMID 29937555

Citations 3

Authors

Di Wang

Yong Jiang

Zhuoran Li

Lu Xue

Xiaoting Li

Yuanjun Liu

Chunli Li

Huiping Wang

Affiliations

Soon will be listed here.

Abstract

Background: The diabetics are prone to skin infections, especially with . It is important to elucidate the different antifungal abilities of patients with hyperglycemia and healthy controls for the treatment of this condition. The toll-like receptor 2 (TLR2) and interleukin (IL)-8 secreted by keratinocytes counteract .

Aim: This study aims to explore the differential expression of toll-like receptor 2 (TLR2) and interleukin (IL)-8 secretion by keratinocytes between controls and diabetic patients when challenged with .

Materials And Methods: HaCaT cells were cultured in high-glucose (HG) Dulbecco's modified Eagle's medium (DMEM) and low-glucose (LG) DMEM. Then, they were exposed to hyphae for 24 h. The expression levels of TLR2 and IL-8 were determined at different periods in both the HG and LG groups. Real-time polymerase chain reaction analysis, western blotting, and enzyme-linked immunosorbent assays were performed in this study. The morphological changes of HaCaT cells under two different glucose concentrations were also observed.

Results: We found that the expression levels of both TLR2 and IL-8 increased and then decreased in the two groups. Notably, the IL-8 levels in the LG group were higher than those in the HG group at each time point ( <0.05), and the TLR2 levels in the LG group were higher than those in the HG group at the beginning of the experiment and after 24 h of treatment with ( <0.05). In each group, the levels of IL-8 and TLR2 at the secretion peak were significantly different from those in the initial and the last period of observation ( <0.05). The cellular morphology of HaCaT cells treated with different concentrations of glucose was also similar. However, with prolonged coculture time, cell death increased.

Conclusion: These observations showed that TLR2 and IL-8 act on the keratinocytes interacting with , and HG status might affect the function of HaCaT cells by reducing the secretion of IL-8 and TLR2.

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